MARKER ASSISTED BREEDING (MAB) FOR SHOOT FLY TOLERANCE IN SORGHUM (Sorghum bicolor (L.) Moench
KrishiKosh
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Title |
MARKER ASSISTED BREEDING (MAB) FOR SHOOT FLY TOLERANCE IN SORGHUM (Sorghum bicolor (L.) Moench
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Creator |
LAKSHMIDEVI, G
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Contributor |
JANILA, P
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Subject |
sorghum, planting, genetics, selection, breeds (animals), genomes, dna, backcrossing, crops, genes
Sorghum bicolor (L.) , Moench |
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Description |
The yield potential of NTJ 2, a popular rabi season sorghum variety of Andhra Pradesh can be enhanced by improving it for shoot fly resistance. This can be achieved through introgression of a QTL on SBI 05 governing leaf glossiness from the donor, J 2779-P4, obtained from ICRISAT using marker assisted backcrossing (MABC). In the present study, foreground and background markers were identified, F1 plants (of the cross, NTJ 2 X J 2779-P 4) were confirmed for hybridity using markers, and 12 F2 plants that are homozygote’s for the target QTL were selected. Thus, the results from this study have set a stage for the proposed MABC program. A set of 17 and 170 SSR primer pairs were evaluated in the parents for identification of foreground and background markers, respectively. Five foreground markers for the target QTL and 35 background markers that distinguish the genome of recipient and donor parent were polymorphic. These polymorphic markers can be used in the MABC. The background markers were distributed on all the 10 chromosomes. The F1 plants derived from the cross, NTJ 2 x J 2779-P4 were confirmed for hybridity using five foreground markers and the confirmed F1 plants were both selfed and backcrossed to NJT 2. A total of 12 plants, homozygotes for the target QTL were selected among 83 F2 plants using the identified foreground markers. The F2 plants were selected, if only all foreground markers tested amplified the donor type alleles (B-type). Further phenotypic selection of these 12 F2 plants is under progress to identify the segregants that are close to NTJ 2 phenotype; they will be advanced to derive both, F3 progenies by selfing and BC1F1 generation by backcrossing to NJT 2.
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Date |
2016-06-07T11:52:29Z
2016-06-07T11:52:29Z 2011 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66915
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Language |
en
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Relation |
D8840;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
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