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Morphological, biochemical and molecular characterization in potato (Solanum tuberosum L.) germplasm

KrishiKosh

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Title Morphological, biochemical and molecular characterization in potato (Solanum tuberosum L.) germplasm
 
Creator Rajani
 
Contributor Singh, Dhirendra
 
Subject plant morphology, biochemistry, molecular biology, characterization, potatoes, solanum tuberosum, germplasm, genotypes, yield components
 
Description Thesis-PhD
The present investigation was conducted during winter season of 2012-13 and 2013-14 at Vegetable Research Centre, G. B. Pant University of Agriculture & Technology, Pantnagar, with fifty genotypes including five checks of potato (Solanum tuberosum L.). The experiment was laid out in an Augmented Block Design having five blocks with fourty-five genotypes and five checks with 60 cm X 20 cm spacing. Each single plot was of 6.0 m2 having 4 rows which were 2 m long. Data were recorded with five randomly selected plants. A total of 17 morphological agronomical and biochemical traits were analysed for genetic variability study, diversity analysis through Principal Component Analysis, Non-hierarchical Euclidean analysis and molecular analysis viz. RAPD and SSR markers.
Analysis of variance revealed significant differences among all the genotypes for all the characters. On basis of yield contributing characters the following genotypes were found superior than the check namely TPSK -05-06-44 (380.80 q/ha), MS/95-1542 (347.12 q/ha), TPSK-05-06- 117 (324.96 q/ha), TPSK-05-06-83 (301.12 q/ha), Kufri Jyoti (298.96 q/ha) and Kufri Pushkar (271.63 q/ha). Stronger positive correlation of total tuber yield was recorded with plant height, number of shoot, number of tuber plant, tuber yield per plot and per cent germination at 30 DAP. Path coefficient analysis revealed that tuber yield per plant had highest positive direct effect on per cent emergence at 30 DAP, plant height, number of shoots per plant, specific gravity, tuber dry matter, number of internodes per stem, internodal length and Protein Content.
All 50 genotypes were classified into non overlapping cluster on the basis of non hierarchical Euclidean cluster analysis. Genotypes were grouped into 8 clusters. High yield per plot, yield per hectare, number of tubers and ascorbic acid content of tuber containing genotypes were presented in cluster IV while high per cent emergence, maximum number of shoots and highest protein content containing genotypes were noted in cluster VII. Cluster VI perceived genotypes having highest mean specific gravity and reducing sugar and cluster II containing genotypes having highest plant height and TSS content. Genotypes belonging to cluster IV, VII and VI were found best in all respects and crossing programme including these genotypes is likely to give best heterotic segregants.
The RAPD and SSR markers used in the study were found to produce polymorphic bands and resulted into differentiation among various genotypes under study. A total of eighty-four loci were amplified by RAPD marker that exhibited 93.00 percent polymorphism. The Jaccard's similarity coefficient range was found to vary from 54 to 100 per cent. SSR amplified thirty-six loci that exhibited 95.45percent polymorphism. Based on the SSR marker data the Jaccard's similarity coefficient was found to vary from 31 to 100 per cent. The results revealed that SSR markers were more efficient having superior polymorphism than RAPD.
 
Date 2016-06-09T12:10:30Z
2016-06-09T12:10:30Z
2015-07
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67096
 
Language en
 
Format application/pdf
 
Publisher G.B. Pant University of Agriculture and Technology, Pantnagar-263145 (Uttarakhand)