Effect of biopriming on seed quality and management of seed borne pathogens in Garden pea (Pisum sativum L.)
KrishiKosh
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Title |
Effect of biopriming on seed quality and management of seed borne pathogens in Garden pea (Pisum sativum L.)
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Creator |
NAIK, MANJUBALA
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Contributor |
KAR, A. K
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Subject |
vegetable crop, seedling, seed borne pathogen, biopriming method
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Description |
An improved seed is a good investment for successful agriculture that demands good germination to produce a vigorous seedling ensuring high yield. Garden pea (Pisum sativum L.) is an important vegetable crop grown during winter season in Odisha. A number of seed borne pathogens affect seed which reduce seed quality and cause seed borne diseases in the field, adversely affecting yield and quality of produce. Present study was carried out to critically analyse quality status of garden pea seeds used by farmers in the state with special references to seed health and to evaluate biopriming methods for managing seed borne diseases and enhancing crop quality in a sustainable way. Thirty-six farmers’ seed samples collected from eight districts were found to possess seed germination 10-15% below IMSCS (i.e. 75%) and carried 40.1-46% fungal count on seeds. Aspergillus flavus, A. niger, Fusarium sp., Penicillium sp., were fungi frequently associated with seeds which caused 24.4-55.6% reduction in germination and caused rotting of seed and seedling shoot and root. Biological agents, Trichoderma viride and Pseudomonas fluorescens were highly effective in inhibiting the growth of pathogens 61.1-81.8% and 83.3-88.8%, respectively in vitro. Seed biopriming with Trichoderma viride (40%) for 4 hour was found the best in enhancing seed quality parameters viz., germination, speed of germination, seedling length, seedling dry weight, SVI-I and SVI-II by 32.8%, 55.4%, 44.5%, 35.3%, 90.7% and 80.2%, respectively and reducing seed rotting by 42.6%. Under green house condition seed biopriming with Trichoderma viride 40% for 4 hour also was best with maximum seedling emergence (85%) and minimum incidence of root rot and seedling blight (9.0% and 8.2%, respectively), biopriming with P. fluorescens being the next best and at par with T. viride. Biopriming also resulted in early initiation of flowering, less days to 50% flowering with enhanced plant height, branches/plant, pods/plant, number of seed/pod, 100 seed weight, dry pod weight and seed yield. Biopriming with Trichoderma viride 40% was the best with figures for above characters 20days, 32days, 38.5cm, 8.2number, 9number, 11number, 36.4g, 7.3g and 36.1g/plant, respectively, and the figure for P. fluorescens (40%) was 20days, 32days, 38.3cm, 8number, 8.8number, 10.8number, 36.0g, 7.2g and 34.2g/plant, respectively, and it was 23.5, 35, 32, 6.6, 6.9, 9.0, 32.0, 5.7g and 19.9g/plant respectively, in control. Biopriming also enhanced chlorophyll content of leaf and increased alpha-amylase activity, carbohydrate and protein content of seed produced which were 62.1, 31.1, 31.7 and 17.2 percent more in T. viride and 47.4, 31.8, 30.4 and 12.2 percent more in P. fluorescens over control. The results thus revealed that seed biopriming with T. viride 40% or P. fluorescens 40% for 4 hr can successfully manage seed borne pathogens and enhance seed and crop quality parameters in garden pea. |
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Date |
2017-01-20T11:41:23Z
2017-01-20T11:41:23Z 2015 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/97302
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Language |
en
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Relation |
Th;4387
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Format |
application/pdf
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