In vitro propagation studies on vanilla (Vanilla planifolia Andr.)
KrishiKosh
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Title |
In vitro propagation studies on vanilla (Vanilla planifolia Andr.)
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Creator |
V.S.Neelannavar
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Contributor |
S.S.Patil
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Subject |
Horticulture
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Description |
An investigation on “in vitro propagation studies on vanilla (vanilla planifolia Andr.).” was carried out during 2003-2005 at the tissue culture laboratory of department of horticulture, college of Agriculture, UAS, Dharwad. In the present investigation five sub experiment were carried out by following CRD design in order to find out best explant, gelling agent, growth regulators for shoot and root initiation and hardening media. Regarding the suitability of explants, axillary bud and shot tip were the best for culture establishment, by producing more number of adventitious shoots with early emergence of primordia. Among the gels used for standardization, sago at 50 g/l + agar 1g/l emerged as the sole replacement of agar, which gave best result in terms of shoot proliferation and subsequent growth. With respect to cost, sago at 50 g/l + agar 1g/l was best replacement for agar which minimized the cost by four times. Among BAP concentration, 1.0 mg/l produced more number of better sized shoots. Among the IBA concentration used, the maximum number of roots with good length in short time were observed on 0.5 mg/l IBA supplemented media. Soil + coco coirpith (1:1) and sand + soil + coco coirpith (1:1:1) medium gave 100% survival percentage at 15 and 45 days after transfer to hardening media and plantlets so hardened were more vigorous. |
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Date |
2016-10-24T17:31:53Z
2016-10-24T17:31:53Z 2005 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/81617
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Format |
application/pdf
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Publisher |
UAS, Dharwad
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