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Studies on salinity tolerance in clusterbean [Cyamopsis tetragonoloba (L.) Taub.] genotypes

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Title Studies on salinity tolerance in clusterbean [Cyamopsis tetragonoloba (L.) Taub.] genotypes
 
Creator Suraj Kala
 
Contributor Goyal, S.C.
 
Subject Genotypes, Planting, Food preservation, Tolerance, Irrigation, Proteins, Germinability, Biological development, Sowing, Amino acids
 
Description The present investigation was aimed to find out the salinity tolerant genotype of clusterbean
[Cyamopsis tetragonoloba (L.) Taub.] using various physiological parameters and to detect the
polypeptide pattern of radicle and plumule of tolerant genotype.
Thirteen genotypes of guar (HG-75, HFG-119, HG-182, HG-258, FS-277, HG-365. HG-563,
HG-867, HG-870, HG-884, HG-2-20, PNB, HG-2-30) were grown in Petri plates containing five
salinity levels viz. 0 (control), 4, 8, 12 and 16 dSm-1. Results revealed that progressive increase of
salinity levels not only decreased the imbibition rate at 2 and 12 h of imbibition but also declined the
per cent seed germination as well as the speed of germination (maguire index). Effective reduction in
seed germination was detected in genotype HG-258 at higher (16 dSm-1) level of salinity. However,
genotype HG-2-20 showed 100 per cent germination at all the levels of salinity.
A progressive increase in salinity levels caused the decrease in plumule length, vigour index,
dry weight and relative water content of radicle as well as plumule while on the other hand radicle
length and dry weight of cotyledonary leaf was enhenced. The maximum enhancement in radicle length
and cotyledonary leaf dry weight was observed in genotype HG-258 and HG-2-20 respectively at higher
level of salinity over control.
An accumulation of metabolites such as proline and total soluble sugar (TSS) in the radicle
and plumule of all the guar genotypes observed with increasing salinity levels. Accumulation of proline
content in radicle and plumule was maximum in genotype HG-563 and HG-2-30 respectively at higher
level (16 dSm-1) of salinity. Highest accumulation of TSS in radicle and plumule was found in
genotype FS-277 and PNB respectively at higher level (16 dSm-1) of salinity over control.
Sodium content of radicle as well as plumule of all the genotypes of guar increased with
increasing salinity level; increase being found to highest in radicle of genotype HG-365 and plumule of
genotype HG-75. Potassium content of radicle as well as plumule showed decreasing trend with
increasing salinity levels. K+/Na+ ratio of radicle and plumule also decreased with increasing salinity
level; decrease being highest in radicle of genotype HG-870 and plumule of genotype HFG-119.
On the basis of the observations taken in the score-card i.e. germination and early seedling
growth, it was found that genotype HG-884 was tolerant to salinity. SDS-PAGE of radicle and plumule
of genotype HG-884 showed new band of 29.86, 72.46, 79.45 and 60.96, 79.45, 92.28 kDa respectively
at all the levels of salinity.
 
Date 2016-11-15T11:19:10Z
2016-11-15T11:19:10Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85556
 
Language en
 
Format application/pdf
 
Publisher CCSHAU