INFLUENCE OF ROOTSTOCKS AND PBR’s ON GROWTH AND PHYSIOLOGY OF APPLE UNDER DIFFERENT SOIL MOISTURE REGIMES
KrishiKosh
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Title |
INFLUENCE OF ROOTSTOCKS AND PBR’s ON GROWTH AND PHYSIOLOGY OF APPLE UNDER DIFFERENT SOIL MOISTURE REGIMES
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Creator |
BHARDWAJ, KAVITA
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Contributor |
CHANDEL, J.S.
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Subject |
rootstocks, planting, irrigation, soil water content, apples, growth substances, grafting, biological development, diseases, amino acids
Stevia rebaudiana Bertoni ,Hills |
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Description |
The present investigation entitled “Studies on major sweet compounds of Stevia rebaudiana Bertoni with plant growth and development under mid hill conditions” was carried out in the experimental field at Nauni and laboratory of the Department of Forest Products, College of Forestry, Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni- Solan (H.P.) during 2008-2009. The research work was based on four experiments. In the 1st experiment i.e. “Extraction and purification of major sweet compounds from Stevia rebaudiana”, the two major compounds (Stevioside and rebaudioside-A) were extracted with methanol from leaves. The analytical method for Stevioside and rebaudioside-A was standardized through HPLC system on amino column using acetonitrile: water (80:20) as the solvent system and Detection was made at 210 nm. In the 2nd experiment i.e. “Studies on variation in major sweet compounds with plant growth and development”, observations were recorded for leaves, green stem and woody stem for fresh weight, dry weight, stevioside and rebaudioside-A contents, stevioside and reabaudioside-A yields per plant at different plant growth and development stages from March to September months. Fresh and dry weights increased from March month onwards and maximized in July month for both leaves (fresh weight-755.90g, dry weight-215.80g) and green stems (fresh weight-414.30g, dry weight-118.50g) whereas woody stem portion showed maximum fresh (620.00g) and dry weights (245.90g) in September month. Maximum stevioside content in leaves (8.55%) was found in June month (vegetative stage) and in green stems (0.82%) and woody stems (0.33%) in September month (flowering stage). Rebaudioside-A content was found maximum in leaves (7.00%) in August (vegetative stage) whereas in green stem (0.93%) and woody stem (0.18%) in September (flowering stage). On single plant basis leaves showed maximum yields of stevioside (17.60g) and rebaudioside-A (13.75g) in July month whereas in green stems and woody stems it was in September month (green stems: 0.83g stevioside and 0.94g rebaudioside-A, Woody stems: 0.82g stevioside and 0.45g rebaudioside-A). On whole plant basis maximum values for fresh weight (1673.00g), dry weight (517.00g), yields of stevioside (18.55g) and rebaudioside-A (14.82 g) were found in of July. Leaf: stem ratio in the samples decreased from 5.31 in March to 0.12 in September.The 3rd experiment was on “Studies on the variation in sweet compounds in different plant parts”. In the open field grown plants, leaves at pre-flowering stage showed maximum stevioside (8.20%) and rebaudioside-A (7.00%) whereas green stems showed maximum content of stevioside (0.82%) and rebaudioside-A (0.93%) at flowering stage. In woody stems, both the sweet compounds lied in a narrow range (stevioside: 0.21-0.33%, rebaudioside-A: 0.15-0.23%).In the polyhouse grown plants, pre-flowering stage showed maximum contents of both the sweet compounds in the leaves (Stevioside-9.19%; rebaudioside-A-5.51%) and green stems (stevioside-0.92%; rebaudioside-A-1.07%). In woody stems both the sweet compounds were in low percentage (stevioside- 0.21-0.27%; rebaudioside-A-0.09-0.39%) in woody stem. In the 4th experiment i.e. “Effect of different drying conditions on percentage of major sweet compounds” the stevioside and rebaudioside-A contents of the leaves dried under open sun, shade and in the oven (at 60oC) did not show significant difference. Signature of Major Advisor Signature |
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Date |
2016-06-16T13:55:49Z
2016-06-16T13:55:49Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/67458
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Language |
en
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Format |
application/pdf
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