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Molecular Studies on Meat Quality Gene in Bandur Sheep

KrishiKosh

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Title Molecular Studies on Meat Quality Gene in Bandur Sheep
 
Creator Sunil Kumar, M.A.
 
Contributor Nagaraja, C.S.
Jayashankar, M.R.
Nadeem Fairoze
Veere Gowda, B.M.
 
Subject Molecular Studies on Meat Quality Gene in Bandur Sheep
 
Description PG. Thesis
A study was conducted with the objective of analyzing the genetic variation at the
CAST region of Bandur breed of sheep by using PCR-RFLP technique. Genomic DNA
was isolated from blood samples of 79 Bandur animals. The genetic diversity of ovine
CAST locus was investigated by PCR-RFLP. A 622 bp fragment of exon 1C/1D from
domain 1 region including the intron was amplified. The size of the amplified product
was same in all the animals studied indicating conservation of DNA at this locus. Two
restriction endonucleases MspI and NcoI were used to detect the genetic variation of the
experimental unit in the CAST gene. CAST/MspI polymorphism was observed as three
patterns which allowed the identification of two alleles viz., A and B. The allelic
frequencies for A and B alleles were 0.74 and 0.26 respectively. The population studied
was in Hardy- Weinberg equilibrium. CAST/NcoI polymorphism also showed three
genotypes with two alleles, viz., A and B, the gene frequencies were 0.697 and 0.303
respectively. The NcoI digested the allele B amplicon, but not allele A. The population
studied was in Hardy- Weinberg equilibrium. The sequence analysis indicated that there
is high homology between the present result and the published ovine CAST sequences.
There was query coverage to the extent of 95%. The present study revealed the genetic
variability at CAST gene locus in Bandur sheep. Further study is suggested to understand
the association of this gene with meat quality traits.
 
Date 2016-11-14T14:59:19Z
2016-11-14T14:59:19Z
2010-09-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85446
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar