Molecular Studies on Meat Quality Gene in Bandur Sheep
KrishiKosh
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Title |
Molecular Studies on Meat Quality Gene in Bandur Sheep
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Creator |
Sunil Kumar, M.A.
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Contributor |
Nagaraja, C.S.
Jayashankar, M.R. Nadeem Fairoze Veere Gowda, B.M. |
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Subject |
Molecular Studies on Meat Quality Gene in Bandur Sheep
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Description |
PG. Thesis
A study was conducted with the objective of analyzing the genetic variation at the CAST region of Bandur breed of sheep by using PCR-RFLP technique. Genomic DNA was isolated from blood samples of 79 Bandur animals. The genetic diversity of ovine CAST locus was investigated by PCR-RFLP. A 622 bp fragment of exon 1C/1D from domain 1 region including the intron was amplified. The size of the amplified product was same in all the animals studied indicating conservation of DNA at this locus. Two restriction endonucleases MspI and NcoI were used to detect the genetic variation of the experimental unit in the CAST gene. CAST/MspI polymorphism was observed as three patterns which allowed the identification of two alleles viz., A and B. The allelic frequencies for A and B alleles were 0.74 and 0.26 respectively. The population studied was in Hardy- Weinberg equilibrium. CAST/NcoI polymorphism also showed three genotypes with two alleles, viz., A and B, the gene frequencies were 0.697 and 0.303 respectively. The NcoI digested the allele B amplicon, but not allele A. The population studied was in Hardy- Weinberg equilibrium. The sequence analysis indicated that there is high homology between the present result and the published ovine CAST sequences. There was query coverage to the extent of 95%. The present study revealed the genetic variability at CAST gene locus in Bandur sheep. Further study is suggested to understand the association of this gene with meat quality traits. |
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Date |
2016-11-14T14:59:19Z
2016-11-14T14:59:19Z 2010-09-15 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/85446
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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