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Evaluation of Non-pathogenic fusarium SPP. for their biological control efficacy against fusarium wilt of watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai)

KrishiKosh

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Title Evaluation of Non-pathogenic fusarium SPP. for their biological control efficacy against fusarium wilt of watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai)
 
Creator B. L, Raghunandan
 
Contributor L, Krishna Naik
 
Subject fungi, pathogens, diseases, planting, enzymes, watermelons, application methods, biological phenomena, fusarium oxysporum, vegetative propagation
 
Description An investigation was carried out to evaluate the efficacy of non-pathogenic Fusarium spp. in the management of Fusarium wilt disease of watermelon. Out of 68 Fusarium cultures isolated from rhizosphere soil of watermelon crop, six Fusarium cultures proved to be non-pathogenic to watermelon and other non-host crops. Morphological, cultural and molecular characterization of non-pathogenic Fusarium cultures was done and among six isolates, five were identified as Fusarium oxysporum and one was Fusarium solani.
Significant inhibition in the growth of the wilt pathogen was showed by non-pathogenic Fusarium cultures UAS NPFu2, UAS NPFu6 and USDA Fo47 in dual culture study. The culture filtrate extracts and volatile compounds of non-pathogenic cultures did not show any significant results on inhibition of the pathogen in vitro. Increased levels of total protein, phenols and defense enzymes such as peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, β-1,3-glucanase and chitinase were observed in the plants treated with non-pathogenic Fusarium cultures. Higher levels of defense enzymes got induced in the plants inoculated with non-pathogenic Fusarium cultures followed by challenged inoculation of pathogen. All the non-pathogenic Fusarium cultures were positive for phosphate solubilization and production of IAA and GA.
Under greenhouse conditions significant increase in plant growth parameters, reduced wilt disease incidence and better root colonization by non-pathogenic Fusarium cultures was observed. The treatments which received non-pathogenic Fusarium + pathogenic Fusarium showed a slight increase in the plant growth parameters compared to inoculation of non-pathogenic culture alone. The isolate UAS NPFu6 performed better than other isolates under field conditions. Seed treatment method gave best results compared to drenching the culture broth of non-pathogenic Fusarium isolates under field study.
 
Date 2016-05-20T13:04:40Z
2016-05-20T13:04:40Z
2013-08-05
 
Type Thesis
 
Identifier Th-10579
http://krishikosh.egranth.ac.in/handle/1/66165
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru