Biological control of phalaris minor in wheat (triticum aestivum L.) using rhizosphere bacteria
KrishiKosh
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Title |
Biological control of phalaris minor in wheat (triticum aestivum L.) using rhizosphere bacteria
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Creator |
Phour, Manisha
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Contributor |
Sindhu, S. S.
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Subject |
Weeds, Biological development, Bacteria, Planting, Wheats, Vegetative propagation, Fungi, Productivity, Herbicides, Drying
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Description |
In the present study, seventy one bacterial cultures were isolated from wheat rhizosphere soil. These bacterial isolates along with twelve reference strains were screened for their effect on seed germination of Phalaris minor on water agar plates. Ten rhizobacterial isolates i.e., HWM1, HWM7, HWM9, HWM11, HWM17, HWM30, HWM37, HWM47, HWM54 and CP43 showed maximum retardation on 5th and 10th of seed germination of Phalaris minor. At 10th day of seed germination, 14.5% bacterial isolates showed retardation of shoot growth and 19.3% bacterial isolates retarded root growth. Out of fifteen rhizobacterial isolates/strains tested for phytotoxicity effect on Phalaris minor, three rhizobacterial isolates/strains i.e., HWM11, P49 and SYB101 only caused yellowing of leaves whereas eight rhizobacterial isolates/strains HWM10, HWM25, KPM15, SB153, PPM126, WPS73, CPA152 and GYB106 caused appearance of disease spot. Screening of rhizobacterial isolates for production of indole acetic acid showed that nine isolates i.e., HWM7, HWM11, HWM18, HWM23, HWM24, HWM37, HWM42, HWM57 and PPM115 produced IAA ranging from 7.0-10.0 Og/ml. Two isolates HWM49 and HWM35 produced 11.10 and 14.07 Og/ml IAA, respectively. Maximum production of IAA (> than 25 Og/ml) was observed in isolates CPS67, CP43 and HWM13. Rhizobacterial isolates/strains HWM59, HWM69, CP43 and CPS67 showed >7.0 Og/ml production of δ-aminolevulinic acid. Other 22 bacterial isolates produced ALA ranging from 2 to 7 Og/ml. Fungal growth inhibition studies showed that 20.5% of total rhizobacterial isolates inhibited the growth of Fusarium oxysporum on PDA medium plates. Large zone of inhibition was formed by bacterial isolates HWM13, WPS73, SB153, HWM25, HWM31, HWM37 and HWM57. Twelve rhizobacterial isolates/strains were tested for their effect on growth of wheat and weed under pot house conditions. Rhizobacterial strains/isolates i.e., SYB101, CPS67 and HWM11 were found to stimulate growth of wheat and inhibited the growth of Phalaris minor. |
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Date |
2016-10-28T09:23:01Z
2016-10-28T09:23:01Z 2012 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/82644
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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