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Molecular diversity of Conogethes spp. Guenèe, (Lepidoptera: Crambidae) infesting castor and cardamom

KrishiKosh

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Title Molecular diversity of Conogethes spp. Guenèe, (Lepidoptera: Crambidae) infesting castor and cardamom
 
Creator VASUDEV, KAMMAR
 
Contributor JAGADISH, P S
 
Subject castor (genus), cardamoms, dna, genetics, ginger, biological phenomena, turmeric, genes, genetic structures, planting
 
Description Study was conducted on genetic diversity of Conogethes species
infesting castor and cardamom based COI, ITS1 and ITS2 gene. The
result of multiple alignments was obtained based on COI gene. Most
number of substitutions were (upto 35) Conogethes found on turmeric
population with castor population (upto 3). The pairwise genetic
distance analysis between the individuals varied from 0.000 to 0.076,
indicating high genetic divergence. The maximum intraspecific pair
wise distance in Conogethes bred on castor was 0.010 then cardamom
population 0.072. The nearest neighbour distance between
Conogethes bred on castor and cardamom was 5.23 per cent,
indicating wide genetic variability between two Conogethes
populations. Sequence length and % G+C content for COI for the
castor population, G+C content showed variations (0.17%) than
cardamom of Conogethes species. The genetic distance within
cardamom population varied from 0.025 to 0.031. This suggests that
the variation in Conogethes population breeding on cardamom at
different location is less. The comparative RAPD banding patterns of
these amplified PCR products from different individuals of different
populations obtained with five primers. The highest distance matrix
between Conogethes specimens on castor population and between
Conogethes specimens of ginger were (0.1951). This indicated that
Conogethes population on castor and cardamom showed significant
genetic variations. In all the above analysis of mtCOI, ITS1 and ITS2
sequences, higher genetic divergence of more than 5 per cent between
Conogethes population on castor and cardamom were obtained. This
suggests that the variation in Conogethes population breeding on
castor and cardamom are genetically heterogeneous.
 
Date 2016-11-11T09:32:24Z
2016-11-11T09:32:24Z
2013-08-23
 
Type Thesis
 
Identifier TH-10515
http://krishikosh.egranth.ac.in/handle/1/85031
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore