Molecular diversity of Conogethes spp. Guenèe, (Lepidoptera: Crambidae) infesting castor and cardamom
KrishiKosh
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Title |
Molecular diversity of Conogethes spp. Guenèe, (Lepidoptera: Crambidae) infesting castor and cardamom
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Creator |
VASUDEV, KAMMAR
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Contributor |
JAGADISH, P S
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Subject |
castor (genus), cardamoms, dna, genetics, ginger, biological phenomena, turmeric, genes, genetic structures, planting
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Description |
Study was conducted on genetic diversity of Conogethes species infesting castor and cardamom based COI, ITS1 and ITS2 gene. The result of multiple alignments was obtained based on COI gene. Most number of substitutions were (upto 35) Conogethes found on turmeric population with castor population (upto 3). The pairwise genetic distance analysis between the individuals varied from 0.000 to 0.076, indicating high genetic divergence. The maximum intraspecific pair wise distance in Conogethes bred on castor was 0.010 then cardamom population 0.072. The nearest neighbour distance between Conogethes bred on castor and cardamom was 5.23 per cent, indicating wide genetic variability between two Conogethes populations. Sequence length and % G+C content for COI for the castor population, G+C content showed variations (0.17%) than cardamom of Conogethes species. The genetic distance within cardamom population varied from 0.025 to 0.031. This suggests that the variation in Conogethes population breeding on cardamom at different location is less. The comparative RAPD banding patterns of these amplified PCR products from different individuals of different populations obtained with five primers. The highest distance matrix between Conogethes specimens on castor population and between Conogethes specimens of ginger were (0.1951). This indicated that Conogethes population on castor and cardamom showed significant genetic variations. In all the above analysis of mtCOI, ITS1 and ITS2 sequences, higher genetic divergence of more than 5 per cent between Conogethes population on castor and cardamom were obtained. This suggests that the variation in Conogethes population breeding on castor and cardamom are genetically heterogeneous. |
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Date |
2016-11-11T09:32:24Z
2016-11-11T09:32:24Z 2013-08-23 |
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Type |
Thesis
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Identifier |
TH-10515
http://krishikosh.egranth.ac.in/handle/1/85031 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences GKVK, Bangalore
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