Record Details

Molecular Detection of Brucella Infection in Livestock and Risk Groups

KrishiKosh

View Archive Info
 
 
Field Value
 
Title Molecular Detection of Brucella Infection in Livestock and Risk Groups
 
Creator Vivekananda
 
Contributor Shrikrishna Isloor
Rathnamma, D.
Veeregowda, B.M.
Venkatesha, M.D.
Prabhudas, K.
Suryanarayana, V.V.S.
Rajeshwari Shome
 
Subject ---
 
Description Ph.D. Thesis
Brucellosis in livestock is a major reproductive disease of economic importance.
It is a major zoonotic disease and occurs as a occupational hazard. Accurate diagnosis
of the disease is essential for institution of disease control strategies. The present study
was undertaken for direct detection of Brucella in clinical samples by PCR. A total of 347
bovines from five herds, 527 sheep from six flocks, 74 pigs from two herds and 99 risk
persons including veterinarians, livestock inspectors and farmers were screened. The
clinical materials viz., blood, serum and vaginal secretion in livestock and blood and
serum in risk persons were collected. The Brucella cultures were isolated in vaginal
swabs from, 18 of 50 cattle, all six buffaloes, six of 33 sheep, four of 24 pigs and blood
sample from one of four human patients, which were serologically positive for brucellosis
in one or more tests. The isolates were identified as B.abortus and B.melitensis by
bacteriological tests and PCR. Among three genus specific primer pairs viz., bcsp 31,
omp2a and omp2b, the bcsp 31 primer pair was found to be more specific and suitable
for confirmation of Brucella isolates by PCR. All the sero negative animals and risk
persons were found negative for brucellosis by isolation and PCR assays. The
polymorphism was detected in omp2a gene of isolates by PCR-RFLP, RE, sequencing
and phylogenetic analysis. The higher percentage of PCR positivity detected in vaginal
samples using bcsp 31 primer pair indicated the suitability of the sample for direct
detection of Brucella by PCR. The species specific primer pairs also detected the
Brucella at species level which could aid in control of brucellosis at species level. In view
of several advantages of PCR such as speed, safety, high sensitivity and specificity over
the conventional methods for the diagnosis of Brucella, the PCR assay can be
considered for routine laboratory diagnosis of brucellosis.
 
Date 2016-07-22T14:58:50Z
2016-07-22T14:58:50Z
2008-12-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69365
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar