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Molecular Diversity of Arbuscular Mycorrhizal Fungi and Pink Pigmented Facultative Methylotrophic Bacteria and their Influence on Grapevine (Vitis Vinifera)

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Title Molecular Diversity of Arbuscular Mycorrhizal Fungi and Pink Pigmented Facultative Methylotrophic Bacteria and their Influence on Grapevine (Vitis Vinifera)
 
Creator P. Jones.Nirmalnath
 
Contributor J.H.Kul;karni
 
Subject Agricultural Microbiology
 
Description Investigations were carried out to study the diversity of native AM fungi and Pink
Pigmented Facultative Methylotroph (PPFM) from four ecological zones of Northern
Karnataka and their growth promoting potentials in grapevine. The highest composition of 23
AMF species was recorded from the Western Ghats. The ITS region of efficient AM fungi
was analysed with data base using BLAST. Based on the results of BLAST the native isolates
AMF 60, 528C, 251SP, 135M, 22A and 235A were grouped in the same cluster of Glomus
mosseae (DQ 400160). The other two isolates viz., G7 and G3 were found to be closely
related to Glomus mosseae (DQ 400141) but formed a separate cluster. The Isolate AMF
T25, has shown close homology to Glomus geosporum (AF 413088).
The authenticity of PPFM isolates was confirmed by screening for the presence of
mxaF gene by amplifying a single fragment of 555 bp in all the native isolates. To understand
the genetic diversity in these isolates, restriction digestion of amplified16S rDNA gene was
done. The sequence analysis of the 16S rDNA has clearly indicated PPFM 365, 120, 61 and
242 to be very close to M. radiotolerance JCM 2831 (D32227). Further, PPFM G4 isolated
from the rhizosphere of vineyards showed close homology with M. fujisawwaense (AJ
250801). PPFM 23 isolate from the saline soils of Northeastern dry zone had very close
homology with M. thiocyanatum (U58018). However, PPFM 117 with MPS activity isolated
from the Western Ghats emerged in a separate clad. The study has brought out the diversity
of AMF and PPFM and their importance in the growth promotional ability in the grapevine.
 
Date 2016-07-23T11:41:01Z
2016-07-23T11:41:01Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69521
 
Format application/pdf
 
Publisher UAS Dharwad