Molecular Genetic Studies on Fec B and Insulin like Growth Factor Genes in Different Sheep Breeds of Karnataka
KrishiKosh
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Title |
Molecular Genetic Studies on Fec B and Insulin like Growth Factor Genes in Different Sheep Breeds of Karnataka
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Creator |
Sudarshan S. Gadad
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Contributor |
Jayashankar, M.R.
Nagaraja, C.S. Satyanarayan, K. Byregowda, S.M. |
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Subject |
Molecular Genetic Studies on Fec B and Insulin Like Growth Factor Genes in Different Sheep Breeds of Karnataka
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Description |
PG. Thesis
A study was conducted with the objective of analyzing the genetic variation of the FecB gene and IGFBP-3 gene region of 50 each of Bannur and Tumkur sheep by using PCR-RFLP technique. Genomic DNA was isolated from blood samples of 50 each of Bannur and Tumkur animals. Approximately 140 bp fragment of exon 6 of FecB gene and 654 bp fragment of exon 2, intron 2, exon 3 and a part of intron 3 region of IGFBP-3 gene were amplified. The sizes of the amplified products were uniform with no variation in size within the breeds of animals studied indicating conservation of DNA at this locus. Restriction endonucleases AvaII and Hinf I were used to detect the genetic variation in the FecB gene and IGFBP-3 gene respectively. AvaII restriction enzyme did not detect any polymorphism, revealing the absence of FecB mutation in Bannur and Tumkur sheep suggesting that all the animals were non-carriers for FecB mutation gene. Hinf I restriction enzyme did not detect any polymorphism in animals studied and yielded a single restriction pattern of 315, 133, 109 and 97 bp revealing monomorphic pattern of IGFBP-3 gene in all the Bannur and Tumkur sheep studied. Two SNPs each at FecB locus, and five SNPs each at IGFBP-3 locus, were identified in Bannur and Tumkur sheep, respectively. Appendices |
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Date |
2016-11-14T15:03:03Z
2016-11-14T15:03:03Z 2011-06-15 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/85447
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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