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Molecular Genetic Studies on Fec B and Insulin like Growth Factor Genes in Different Sheep Breeds of Karnataka

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Title Molecular Genetic Studies on Fec B and Insulin like Growth Factor Genes in Different Sheep Breeds of Karnataka
 
Creator Sudarshan S. Gadad
 
Contributor Jayashankar, M.R.
Nagaraja, C.S.
Satyanarayan, K.
Byregowda, S.M.
 
Subject Molecular Genetic Studies on Fec B and Insulin Like Growth Factor Genes in Different Sheep Breeds of Karnataka
 
Description PG. Thesis
A study was conducted with the objective of analyzing the genetic variation of the
FecB gene and IGFBP-3 gene region of 50 each of Bannur and Tumkur sheep by using
PCR-RFLP technique. Genomic DNA was isolated from blood samples of 50 each of
Bannur and Tumkur animals. Approximately 140 bp fragment of exon 6 of FecB gene
and 654 bp fragment of exon 2, intron 2, exon 3 and a part of intron 3 region of IGFBP-3
gene were amplified. The sizes of the amplified products were uniform with no variation
in size within the breeds of animals studied indicating conservation of DNA at this locus.
Restriction endonucleases AvaII and Hinf I were used to detect the genetic variation in
the FecB gene and IGFBP-3 gene respectively. AvaII restriction enzyme did not detect
any polymorphism, revealing the absence of FecB mutation in Bannur and Tumkur sheep
suggesting that all the animals were non-carriers for FecB mutation gene. Hinf I
restriction enzyme did not detect any polymorphism in animals studied and yielded a
single restriction pattern of 315, 133, 109 and 97 bp revealing monomorphic pattern of
IGFBP-3 gene in all the Bannur and Tumkur sheep studied. Two SNPs each at FecB
locus, and five SNPs each at IGFBP-3 locus, were identified in Bannur and Tumkur
sheep, respectively.
Appendices
 
Date 2016-11-14T15:03:03Z
2016-11-14T15:03:03Z
2011-06-15
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85447
 
Language en
 
Format application/pdf
 
Publisher Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar