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Cloning and Expression Analysis of HSP 101 Gene In Bread Wheat (Triticum aestivum L.Em.Thell.)
KrishiKosh
View Archive Info| Field | Value | |
| Title |
Cloning and Expression Analysis of HSP 101 Gene In Bread Wheat (Triticum aestivum L.Em.Thell.)
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| Creator |
Singal, Manjjri
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| Contributor |
Dhillon, Santosh
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| Subject |
wheats, genes, planting, tolerance, proteins, transcription, biological phenomena, genotypes, grain, triticum aestivum
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| Description |
The four genotypes/varieties of wheat i.e. two heat tolerant (WH730 and WH 1021) and two heat sensitive (WH 147 and WH 1021) varieties were raised in field under normal and late sown conditions and data on morpho-phsiological traits like canopy temperature depression, grain yield, 100 seed weight and heat susceptibility index were evaluated to assess the effect of high temperature on these traits. High temperature negatively affected all these traits. The affect was more in thermosensitive varieties as compared to thermotolerant varieties. The order of thermotolerance based on heat susceptibility index (HSI) was: WH 730 (0.42) > WH 1021 (0.68) > WH 711 (1.19) > WH 147 (1.53). Expression analysis of HSP101transcripts (HSP101, HSP101band HSP101c) were studied by growing the above four varieties in controlled conditions where artificial heat stress at different temperatures (25º, 31ºC, 34ºC, 37ºC and 40ºC) were given to plants at the seedling stage. All heat shock transcripts (HSP101, 101b & 101c) were found to be expressed at all heat shock treatments and were found to be maximum at 40ºC. In all heat shock transcripts (HSP101, 101b & 101c) WH730 showed the maximum level of gene expression and WH 147 showed minimum level of gene expression. Among all the three HSP transcripts, (HSP101, HSP101b and HSP101c) expression level of HSP101 was found to be maximum in all the four varieties. Partial cDNA sequences of HSP101 (1066 bp), HSP101b (1077 bp) and HSP101c (1249 bp) were cloned and sequenced from WH 730 (heat tolerant) and WH 147 (heat sensitive) genotypes. Phylogram generated using gene sequences of HSP101 isoforms (HSP101, HSP101b and HSP101c ) of WH 730 and WH 147, gene sequences of HSP101 isoforms of Triticum aestivum and other crop plants (Triticum turgidum, Zea mays, and Oryza sativa) available in Genbank, demonstrated that all the sequences are descendents of a common ancestor. Sequences of HSP101 gene and sequences of HSP101b gene were found to be closest in comparison to sequences of HSP101c gene. WH 730 showed maximum thermotolerance while WH 147 was least thermotolerant, based on overall picture of morpho-phsiological traits and gene expression analysis studies. |
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| Date |
2016-02-09T11:58:11Z
2016-02-09T11:58:11Z 2014 |
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| Type |
Thesis
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| Identifier |
http://krishikosh.egranth.ac.in/handle/1/64234
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| Language |
en
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| Format |
application/pdf
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| Publisher |
CCSHAU
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