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Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes

KrishiKosh

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Title Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes
 
Creator Singh, U. A.
 
Contributor Kalorey, D. R.
 
Subject Animal Biotechnology
Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes
 
Description In the present investigation, genetic diversity among L. monocytogenes
isolates recovered from foods of animal origin (milk, milk product, meat and meat
products), vegetables and human clinical cases with bad obstetrics history was
studied by employing the RAPD- PCR. Total of 259 samples were screened for
the isolation and identification of L. monocytogenes. The isolation was attempted by two-step enrichment procedure in UVM-1 and 2 enrichment broths followed by
plating on DRIA. Confirmation of the isolates was based on biochemical tests,
CAMP test, haemolysis on sheep blood agar and PI-PLC activity.
Overall, prevalence of L. monocytogenes spp. in the present investigation
has been recorded 11.1%, of these, 5 (19.2 %) isolates were confirmed as L.
monocytogenes from vegetables, 14 (17.5 %) from milk, 2 (9.5 %) from milk
product,3 (5.7 %) from meat,1 (8.3 %) from meat product and 4(5.8%) from
humans clinical cases with bad obstetrics histroy.
Serotyping PCR study revealed that 29 L. monocytogenes isolates
recovered from different sources were found to be of serotype 1/2b and 1/2b, 4b..
In conclusion 1/2b & 4b pathogenic L. monocytogenes exist in food of animal
origin as well as human clinical cases with bad obstetrics history.
Cluster dendrogram prepared from RAPD profile of 29 L. monocytogenes
resulted into 5 clusters indicating five major RAPD pattern of L. monocytogenes .
All clusters include the isolates from various sources except cluster 3 and 5 which
included isolates from human and milk origin, respectively. RAPD pattern 4 and 5
includes the isolates belonging to 1/2b, 4b serovar, whereas, other pattern,
include L. monocytogenes belonging to various serovars
In conclusion prevalance of pathogenic L. monocytogenes in different
samples signifies the zoonotic potential. Serotyping and RAPD PCR offers
greater discrimination of Listeria strain.
 
Date 2017-01-03T17:01:19Z
2017-01-03T17:01:19Z
2012-09-16
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94278
 
Language en
 
Format application/pdf
 
Publisher MAFSU