Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes
KrishiKosh
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Title |
Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes
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Creator |
Singh, U. A.
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Contributor |
Kalorey, D. R.
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Subject |
Animal Biotechnology
Random Amplified Polymorphic DNA (RAPD) Analysis Of Listeria Monocytogenes |
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Description |
In the present investigation, genetic diversity among L. monocytogenes isolates recovered from foods of animal origin (milk, milk product, meat and meat products), vegetables and human clinical cases with bad obstetrics history was studied by employing the RAPD- PCR. Total of 259 samples were screened for the isolation and identification of L. monocytogenes. The isolation was attempted by two-step enrichment procedure in UVM-1 and 2 enrichment broths followed by plating on DRIA. Confirmation of the isolates was based on biochemical tests, CAMP test, haemolysis on sheep blood agar and PI-PLC activity. Overall, prevalence of L. monocytogenes spp. in the present investigation has been recorded 11.1%, of these, 5 (19.2 %) isolates were confirmed as L. monocytogenes from vegetables, 14 (17.5 %) from milk, 2 (9.5 %) from milk product,3 (5.7 %) from meat,1 (8.3 %) from meat product and 4(5.8%) from humans clinical cases with bad obstetrics histroy. Serotyping PCR study revealed that 29 L. monocytogenes isolates recovered from different sources were found to be of serotype 1/2b and 1/2b, 4b.. In conclusion 1/2b & 4b pathogenic L. monocytogenes exist in food of animal origin as well as human clinical cases with bad obstetrics history. Cluster dendrogram prepared from RAPD profile of 29 L. monocytogenes resulted into 5 clusters indicating five major RAPD pattern of L. monocytogenes . All clusters include the isolates from various sources except cluster 3 and 5 which included isolates from human and milk origin, respectively. RAPD pattern 4 and 5 includes the isolates belonging to 1/2b, 4b serovar, whereas, other pattern, include L. monocytogenes belonging to various serovars In conclusion prevalance of pathogenic L. monocytogenes in different samples signifies the zoonotic potential. Serotyping and RAPD PCR offers greater discrimination of Listeria strain. |
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Date |
2017-01-03T17:01:19Z
2017-01-03T17:01:19Z 2012-09-16 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/94278
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Language |
en
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Format |
application/pdf
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Publisher |
MAFSU
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