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Functional and Molecular diversity of Fluorescent Pseudomonads from Western Ghat regions of Uttara Kannada District

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Title Functional and Molecular diversity of Fluorescent Pseudomonads from Western Ghat regions of Uttara Kannada District
 
Creator Y.J.Megha
 
Contributor A.R.Alagawadi
 
Subject Agricultural Microbiology
 
Description from Western Ghats of Uttara Kannada district, Karnataka. Thirty seven of
them were tentatively identified as Pseudomonas fluorescens, 13 as Pseudomonas aeruginosa
and two isolates as Pseudomonas aureofaciens based on the morphological, biochemical and
physiological properties. Out of the 52 isolates, 44 were inhibitory to one or the other plant
pathogens tested. While14 were inhibitory to R. solanacearum, 10 were inhibitory to X.
campestris, 32 to F. oxysporum and 24 to R. bataticola.Twenty two isolates showed
inhibition against 2-3 pathogens. All the 44 isolates with biocontrol activity showed HCN
production including 15 high HCN producers. Ten isolates tested for antimicrobial metabolite
production showed production of one to two metabolites some of which were found to
inhibit X. campestris, F. oxysporum and R. bataticola.
The amount of Pi released from TCP ranged from 1.78 to 15.44 per cent where as the
amount of IAA and GA produced ranged from 80 to 760 μg/l and 24.8 to 262.8 μg/l of broth
respectively. Forty seven of them recorded the alkaline protease activity in the range of 0.50
to 6.18 mg tyrosine/mg protein/hour.
Molecular diversity of fifteen isolates along with five reference strains was analyzed
by RAPD-PCR. The dendrogram constructed had six clusters with maximum genetic
similarity of 0.73 and minimum genetic similarity of 0.00.
The diversity analysis of 52 fluorescent pseudomonad isolates using diversity indices
for the pooled data showed higher Shannon’s index of 0.67 suggesting their high diversity in
the study area. An evenness index of 0.61 indicates the dominance of one species over
others. A low richness index suggested unequal distribution of fluorescent pseudomonads in
different forests.
 
Date 2016-10-17T10:49:14Z
2016-10-17T10:49:14Z
2005
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/80660
 
Format application/pdf
 
Publisher UAS, Dharwad