Functional and Molecular diversity of Fluorescent Pseudomonads from Western Ghat regions of Uttara Kannada District
KrishiKosh
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Title |
Functional and Molecular diversity of Fluorescent Pseudomonads from Western Ghat regions of Uttara Kannada District
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Creator |
Y.J.Megha
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Contributor |
A.R.Alagawadi
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Subject |
Agricultural Microbiology
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Description |
from Western Ghats of Uttara Kannada district, Karnataka. Thirty seven of them were tentatively identified as Pseudomonas fluorescens, 13 as Pseudomonas aeruginosa and two isolates as Pseudomonas aureofaciens based on the morphological, biochemical and physiological properties. Out of the 52 isolates, 44 were inhibitory to one or the other plant pathogens tested. While14 were inhibitory to R. solanacearum, 10 were inhibitory to X. campestris, 32 to F. oxysporum and 24 to R. bataticola.Twenty two isolates showed inhibition against 2-3 pathogens. All the 44 isolates with biocontrol activity showed HCN production including 15 high HCN producers. Ten isolates tested for antimicrobial metabolite production showed production of one to two metabolites some of which were found to inhibit X. campestris, F. oxysporum and R. bataticola. The amount of Pi released from TCP ranged from 1.78 to 15.44 per cent where as the amount of IAA and GA produced ranged from 80 to 760 μg/l and 24.8 to 262.8 μg/l of broth respectively. Forty seven of them recorded the alkaline protease activity in the range of 0.50 to 6.18 mg tyrosine/mg protein/hour. Molecular diversity of fifteen isolates along with five reference strains was analyzed by RAPD-PCR. The dendrogram constructed had six clusters with maximum genetic similarity of 0.73 and minimum genetic similarity of 0.00. The diversity analysis of 52 fluorescent pseudomonad isolates using diversity indices for the pooled data showed higher Shannon’s index of 0.67 suggesting their high diversity in the study area. An evenness index of 0.61 indicates the dominance of one species over others. A low richness index suggested unequal distribution of fluorescent pseudomonads in different forests. |
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Date |
2016-10-17T10:49:14Z
2016-10-17T10:49:14Z 2005 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/80660
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Format |
application/pdf
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Publisher |
UAS, Dharwad
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