Biological and Molecular characterization and management of watermelon bud necrosis virus
KrishiKosh
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Title |
Biological and Molecular characterization and management of watermelon bud necrosis virus
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Creator |
T.Rajasekharam
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Contributor |
M.S.Patil
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Subject |
Plant Pathology
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Description |
Survey carried out during 2006-08 revealed that the Watermelon bud necrosis virus (WBNV) was prevalent in Karnataka and Andhra Pradesh causing bud necrosis disease in watermelon, muskmelon, bitter gourd, ridge gourd and bottle gourd. The WBNV incidence in Karnataka ranged from 0 to 100 per cent and in Andhra Pradesh it was 0 to 80 per cent. Maximum concentration of the virus was observed in leaves. Virus was detected up to 1:1000 dilutions in DBIA. WBNV was transmitted by sap using 2-mercaptoethanol in 0.05 M potassium phosphate buffer (pH 7.0), to Cowpea cv. C-152. Quasi spherical virus particles measuring 80-100 nm in diameter was observed under electron microscope. WBNV produced symptoms like chlorotic and necrotic spots on leaves, necrosis and dieback of buds and chlorotic and necrotic rings on fruit. WBNV could infect 22 host plant species belonging to 15 genera in six families by mechanical sap inoculations. WBNV was successfully transmitted by Thrips palmi. On the basis of NP gene and protein sequence relationship, two tospovirus were identified, namely WBNV and GBNV. The sequenced region in WBNV and GBNV isolates contained a single open reading frame of 828 and 831 bases, respectively. Out of 20 cultivars screened against WBNV, none recorded resistance. Integrated disease management strategies was developed involving combination of practices viz., drip irrigation, seed treatment with Imidacloprid (5 g/kg), use of silver colour UV reflective mulch and alternate sprays of Spinosad (0.3 ml/l), Imidacloprid (0.3 ml/l) and Thiomethoxam (0.2 g/l) including bunds and keeping plot weed free up to flowering recoded least mean PDI (0.21%). |
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Date |
2016-07-25T10:44:55Z
2016-07-25T10:44:55Z 2010 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/69674
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Format |
application/pdf
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Publisher |
UAS Dharwad
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