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Biological and Molecular characterization and management of watermelon bud necrosis virus

KrishiKosh

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Title Biological and Molecular characterization and management of watermelon bud necrosis virus
 
Creator T.Rajasekharam
 
Contributor M.S.Patil
 
Subject Plant Pathology
 
Description Survey carried out during 2006-08 revealed that the Watermelon bud necrosis virus
(WBNV) was prevalent in Karnataka and Andhra Pradesh causing bud necrosis disease in
watermelon, muskmelon, bitter gourd, ridge gourd and bottle gourd. The WBNV incidence in
Karnataka ranged from 0 to 100 per cent and in Andhra Pradesh it was 0 to 80 per cent.
Maximum concentration of the virus was observed in leaves. Virus was detected up to
1:1000 dilutions in DBIA. WBNV was transmitted by sap using 2-mercaptoethanol in 0.05 M
potassium phosphate buffer (pH 7.0), to Cowpea cv. C-152. Quasi spherical virus particles
measuring 80-100 nm in diameter was observed under electron microscope. WBNV produced
symptoms like chlorotic and necrotic spots on leaves, necrosis and dieback of buds and
chlorotic and necrotic rings on fruit. WBNV could infect 22 host plant species belonging to
15 genera in six families by mechanical sap inoculations. WBNV was successfully
transmitted by Thrips palmi.
On the basis of NP gene and protein sequence relationship, two tospovirus were
identified, namely WBNV and GBNV. The sequenced region in WBNV and GBNV isolates
contained a single open reading frame of 828 and 831 bases, respectively.
Out of 20 cultivars screened against WBNV, none recorded resistance. Integrated
disease management strategies was developed involving combination of practices viz., drip
irrigation, seed treatment with Imidacloprid (5 g/kg), use of silver colour UV reflective mulch
and alternate sprays of Spinosad (0.3 ml/l), Imidacloprid (0.3 ml/l) and Thiomethoxam (0.2
g/l) including bunds and keeping plot weed free up to flowering recoded least mean PDI
(0.21%).
 
Date 2016-07-25T10:44:55Z
2016-07-25T10:44:55Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69674
 
Format application/pdf
 
Publisher UAS Dharwad