Studies on Molecular Variations in Puccinia arachidis Speg. Causing Rust of Groundnut
KrishiKosh
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Title |
Studies on Molecular Variations in Puccinia arachidis Speg. Causing Rust of Groundnut
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Creator |
C.B. Tashildar
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Contributor |
S.S. Adiver
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Subject |
Plant pathology
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Description |
An investigation was carried out to study morphological and molecular variation in Puccinia arachidis Speg. Five isolates were collected from different varieties grown in MARS, Dharwad and ten isolates from other locations. Morphological variation was studied by considering uredospore morphology. Molecular variation was studied by PAGE technique using selected isozymes and RAPD technique using ten random primers. In morphological variation, the dimension of uredospores of all isolates was in the range of 21-26 × 18-21 μm. Nearly all spores had light cinnamon colour and globose to ovoid shape. The pits on the surface of the spores were observed in Annigere, Bellary, Jalgaon and Kolhapur isolates only. The echinulations were scarce to fine in all isolates. A teleospore measuring 30.64 x 19.33 μm was found in the Gadag isolate. This is a rare occurrence and needs further study. PAGE analysis of Peroxidase revealed two distinct groups in Dharwad isolates. Dh-86, Dh-101, GPBD-4 and JL-24 isolates formed one group whereas and TAG-24 isolate formed another group. In other locations, Gadag isolate formed a distinct group. Other isolates formed another group. Polyphenol oxidase analysis too revealed two distinct groups in Dharwad isolates. Dh-86, Dh-101 and JL- 24 isolates formed one group whereas GPBD-4 and TAG-24 isolates formed another group. In other locations too, Jalgaon isolate formed a distinct group. Other isolates formed another group. In case of molecular variation with the RAPD markers, OPD-18 (5’- GAGAGCCAAC-3’) gave highest amplification. RAPD analysis revealed two distinct groups within Dharwad isolates. Dh-86, Dh-101 and TAG-24 isolates formed one group. GPBD-4 and JL-24 isolates formed another group. In other locations, Dharwad and Raichur formed a distinct group. Rest of the isolates formed another group. Isozyme studies with PAGE and RAPD revealed minor variation in the pathogen in spite of insignificant morphological variation; which is useful to understand host-pathogen interaction. |
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Date |
2016-11-11T16:55:00Z
2016-11-11T16:55:00Z 2011 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/85185
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Format |
application/pdf
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Publisher |
UAS, Dharwad
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