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Genome-wide molecular mapping, introgression of stable QTLs and expressional quantitation of transcription factor genes in charcoal rot manifestation in sorghum bicolour (L) Moench

KrishiKosh

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Title Genome-wide molecular mapping, introgression of stable QTLs and expressional quantitation of transcription factor genes in charcoal rot manifestation in sorghum bicolour (L) Moench
 
Creator Ayyanagouda.M.Patil
 
Contributor B.Fakrudin
 
Subject Plant Bio - Technology
 
Description Recombinant inbred lines derived from the cross IS22380 (susceptible) and E36-1
(resistant) were characterized for the morphological and biochemical components of charcoal
rot resistance and yield related traits at three locations over four rabi seasons revealed
significant differences among RILs and GxE interactions. A significant association among
relevant traits was noticed at phenotypic and genotypic level with high heritability estimates.
A total of 141 polymorphic DNA markers (48/275 SSRs, 65/533 EST-SSRs, 28/410 RAPD)
were genotyped to construct a genetic linkage map spanning 2905 cM and used for
Composite Interval Mapping of QTLs. Stable QTLs were detected lodging percent (xtxp176–
xtxp312 (CR1) number of internodes crossed by the fungus (xtxp297-xiabt273 (CR2) and
length of infection (xtxp275-xtxp241(CR3): the additive effect at all these loci was
contributed by E36-1. Two major QTLs of plant height and three QTLs of plant yield
accounted for 38% and 41% phenotypic variance respectively. On LG-I, the genomic region
flanked by xtxp274–xiabt29 harbored a common QTL. Three stable QTLs, CR1, CR2 and
CR3 collectively contributing 43% of phenotypic variation were introgressed into M35-1 and
SPV86 backgrounds: at BC1F1 with 2285 and 2033 marker and at BC2F1 5989 and 6002
marker points in M35-1 and SPV86 backgrounds were screened to identify progenies
harboring all the three QTLs.
Expressional quantitation of 185 transcription factor genes was done in charcoal rot
challenged and control tissue of E36-1 and SPV86 genotypes at 75 DAS and 90 DAS where
142 genes up regulated in both genotypes while eight genes of five family viz., AP2
(PTSb00019.1, PTSb00022.1, PTSb00024.1 WRKY (PTSb00331.1), HMG (PTSb00179.1,
PTSb00185.1) ARF (PTSb00033.1) and bHLH (PTSb00349.1) were up regulated in
pathogen challenged tissues. Two genes of WRKY family (PTSb00328.1 and PTSb00331.1)
were significantly up regulated in resistant genotype alone: these understandings would help
in developing strategies for resistance breeding in sorghum.
 
Date 2016-07-23T10:53:05Z
2016-07-23T10:53:05Z
2009
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/69501
 
Format application/pdf
 
Publisher UAS Dharwad