Development and Exploitation of Heterotic pools of Hirsutum and Barbadense for Developing Potential Inter Specific Hybrods, Molecular Marker and Genetic Transformation Study in Cotton.
KrishiKosh
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Title |
Development and Exploitation of Heterotic pools of Hirsutum and Barbadense for Developing Potential Inter Specific Hybrods, Molecular Marker and Genetic Transformation Study in Cotton.
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Creator |
Yanal Alkuddsi
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Contributor |
S. S. Patil
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Subject |
Genetics & Plant Breeding
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Description |
Reciprocal selection for improving combining ability was modified to suit cotton and tested. Based on earlier studies DB 533 and DB 534 the selected barbadense elite combiners were crossed and advanced to F4 generation for creating recombinational variability for combining ability against the hirsutum testers (DH 98-27, ZCH 8, 178-24 and DH 18-31). In line x tester study, DB 533 and DB 534 were compared with barbadense lines in developing productive inter specific hybrids. Both of them recorded positive gca effects for seed cotton yield confirming potentiality to form heterotic groups of hirsutum vs barbadense cottons. Of the 23 hirsutum testers, four testers mentioned above exhibited positive gca values for yield. The potentiality of the heterotic box was also confirmed by comparing these eight bench mark crosses with 49 inter specific crosses and checks. Among the 53 F5 barbadense lines, DB 533 x DB 534 F5 IPS 18 showed exceptional superiority for productivity and fiber quality traits. Among three methods of determining pooled score, weighted percent gca approach was efficient in identification of potential combiners. Based on mean and coefficient of variability for productivity DH 98-27 was found to be efficient tester. Sub grouping the F4 lines against pairs of hirsutum testers was done. Among the population used four F4 lines revealed transgressive positive segregation for combining ability against all the four testers and the lines DB 533 x DB 534 F4 IPS 49 and DB 534 x DB 533 F4 IPS 22 were selected for developing sub populations against hirsutum testers. With the help of 40 SSR markers, molecular diversity was assessed and positive correlation was found between genetic distance (GD) and seed cotton yield of F1 and heterosis over checks. In vivo transformation was attempted in hirsutum variety. |
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Date |
2016-07-26T10:22:56Z
2016-07-26T10:22:56Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/69838
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Format |
application/pdf
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Publisher |
UAS Dharwad
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