Overexpression of gene encoding citrate synthase in Pigeopea (Cajanus cajan (L.) Mill sp) to enhance phosphorus uptake under phosphorus deficient conditions
KrishiKosh
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Title |
Overexpression of gene encoding citrate synthase in Pigeopea (Cajanus cajan (L.) Mill sp) to enhance phosphorus uptake under phosphorus deficient conditions
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Creator |
R, Krishnappa
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Contributor |
I.S, Aftab hussain
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Subject |
planting, vegetative propagation, transgenics, organic acid salts, genes, genetic processes, exudates, acidity, pigeonpeas, selection
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Description |
Plants have adopted several strategies, to take up phosphorus (P) from the soil under P deficient conditions. Exudation of Organic acids like citrate is one such strategy where in the released citrate solubilizes the soil P bound to different elements and makes it available to plant. Hence attempt was made to develop transgenic pigeon pea plants overexpressing citrate synthase(CS) gene to enhance citrate production. Putative Pigeonpea transgenic lines overexpressing citrate synthase gene were developed using Inplanta method of transformation. Kanamycin screening, PCR analysis and protein expression techniques were used to confirm the successful integration and expression of CS gene. The putative transgenic pigeon pea plants showing increased tissue citrate synthase content compare to wildtype in T1 and T2 generation were selected. In T3 generation, physiological and biochemical studies were conducted to screen the putative transgenic lines for enhanced P uptake. Increased citrate synthase content in root and leaf was observed in selected transgenic lines leading to increased phosphorus accumulation in the leaf. Under P deficient conditions root length, root surface area and root to shoot ratio were more compare to wildtype. Under P deficient conditions, selected transgenic plants showed an increase in root citrate synthase content and root citrate content compared to P sufficient conditions. The shoot growth of transgenic pigeon pea plants was also more compared to wildtype under phosphorus deficient conditions. The photosynthetic rate of transgenic plants was higher compare to wildtype under P deficient conditions. The increased root citrate synthase content of transgenic plants was correlated to increased citrate content and tissue phosphorus accumulation. Molecular analysis of putative transgenic pigeon pea plants confirmed the successful integration and expression of citrate synthase gene. |
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Date |
2016-05-17T09:46:16Z
2016-05-17T09:46:16Z 2011-09-22 |
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Type |
Thesis
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Identifier |
Th-10153
http://krishikosh.egranth.ac.in/handle/1/66000 |
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Language |
en
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Format |
application/pdf
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Publisher |
University of Agricultural Sciences, Bengaluru
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