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Overexpression of gene encoding citrate synthase in Pigeopea (Cajanus cajan (L.) Mill sp) to enhance phosphorus uptake under phosphorus deficient conditions

KrishiKosh

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Title Overexpression of gene encoding citrate synthase in Pigeopea (Cajanus cajan (L.) Mill sp) to enhance phosphorus uptake under phosphorus deficient conditions
 
Creator R, Krishnappa
 
Contributor I.S, Aftab hussain
 
Subject planting, vegetative propagation, transgenics, organic acid salts, genes, genetic processes, exudates, acidity, pigeonpeas, selection
 
Description Plants have adopted several strategies, to take up phosphorus (P) from the soil
under P deficient conditions. Exudation of Organic acids like citrate is one such strategy
where in the released citrate solubilizes the soil P bound to different elements and makes
it available to plant. Hence attempt was made to develop transgenic pigeon pea plants
overexpressing citrate synthase(CS) gene to enhance citrate production.
Putative Pigeonpea transgenic lines overexpressing citrate synthase gene were
developed using Inplanta method of transformation. Kanamycin screening, PCR analysis
and protein expression techniques were used to confirm the successful integration and
expression of CS gene. The putative transgenic pigeon pea plants showing increased
tissue citrate synthase content compare to wildtype in T1 and T2 generation were selected.
In T3 generation, physiological and biochemical studies were conducted to screen the
putative transgenic lines for enhanced P uptake. Increased citrate synthase content in root
and leaf was observed in selected transgenic lines leading to increased phosphorus
accumulation in the leaf. Under P deficient conditions root length, root surface area and
root to shoot ratio were more compare to wildtype. Under P deficient conditions, selected
transgenic plants showed an increase in root citrate synthase content and root citrate
content compared to P sufficient conditions. The shoot growth of transgenic pigeon pea
plants was also more compared to wildtype under phosphorus deficient conditions. The
photosynthetic rate of transgenic plants was higher compare to wildtype under P deficient
conditions. The increased root citrate synthase content of transgenic plants was correlated
to increased citrate content and tissue phosphorus accumulation. Molecular analysis of
putative transgenic pigeon pea plants confirmed the successful integration and expression
of citrate synthase gene.
 
Date 2016-05-17T09:46:16Z
2016-05-17T09:46:16Z
2011-09-22
 
Type Thesis
 
Identifier Th-10153
http://krishikosh.egranth.ac.in/handle/1/66000
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru