STUDIES ON MOLECULAR CHARACTERIZATION OF A GEMINIVIRUS AND ITS INTERACTION WITH ANTIVIRAL COMPOUNDS
KrishiKosh
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Title |
STUDIES ON MOLECULAR CHARACTERIZATION OF A GEMINIVIRUS AND ITS INTERACTION WITH ANTIVIRAL COMPOUNDS
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Creator |
VERMA, YASHVEER SINGH
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Contributor |
BHARDWAJ, S.V.
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Subject |
diseases, vegetables, dna, proteins, viruses, biological phenomena, planting, genes, genetic structures, genomes
Geminiviridae |
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Description |
ABSTARCT Geminiviruses are small isometric particles which contain either one or two circular single stranded DNAs. The family Geminiviridae comprises of four genera: Mastrevirus, Curtovirus, Topocuvirus and Begomovirus. Among begomoviruses, one of the most important virus is TYLCV which infects an economically important food crop i.e. tomato (Lycopersicon esculentum), all over the world. TYLCV is also prevalent in different regions of India including HP. Five samples from different tomato growing areas of district Solan (HP) were collected for serological detection of TYLCV using DAS-ELISA. All samples reacted positively with the antisera. In further confirmation studies, CP (coat protein) gene was amplified from Tohana and Manjhed isolates, using CP gene specific primers. A 771 bp long CP sequence was amplified, sequenced and submitted to NCBI database (Acc no-KC253231). TYLCV, the test virus was confirmed to be a begomovirus on the basis of phylogenetic analysis while comparing it with other geminiviruses. On comparison with other Indian tomato leaf curl viral isolates, it was found to be closely related to Dharwad isolate (GI 1160428) and not to Palampur isolate (GI 1244840), which indicates it’s origin in other than North Indian region and evolution of a new viral strain, though it showed 70 to >90 per cent resemblance with North Indian isolates. To predict the structure of TYLCV coat protein, ab initio approach was followed as no structural homolog was found during database search. AAVP (Acc no-JQ 347294) was selected to be tested as putative inhibitor of TYLCV. AAVP structure was predicted based on similarity search with bougainvillea template. Other antimicrobial proteins; magainin, cryptdin, moricin and Ribonuclease were also tested to check their effectiveness against TYLCV, based on literature serach for antimicrobial agents. Ligand-receptor interaction were performed using online server “Patchdock”. Out of five antimicrobial proteins tested, AAVP was found to be most effective and potential viral inhibitor with highest interaction score (14156), largest active site coverage (2455.20) with ACE value of 134.33. Based on these studies AAVP is predicted to act as a potent inhibitor of geminiviruses. |
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Date |
2016-05-31T15:30:37Z
2016-05-31T15:30:37Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/66497
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Language |
en
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Format |
application/pdf
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