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STUDIES ON MOLECULAR CHARACTERIZATION OF A GEMINIVIRUS AND ITS INTERACTION WITH ANTIVIRAL COMPOUNDS

KrishiKosh

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Title STUDIES ON MOLECULAR CHARACTERIZATION OF A GEMINIVIRUS AND ITS INTERACTION WITH ANTIVIRAL COMPOUNDS
 
Creator VERMA, YASHVEER SINGH
 
Contributor BHARDWAJ, S.V.
 
Subject diseases, vegetables, dna, proteins, viruses, biological phenomena, planting, genes, genetic structures, genomes
Geminiviridae
 
Description ABSTARCT
Geminiviruses are small isometric particles which contain either one or two circular single
stranded DNAs. The family Geminiviridae comprises of four genera: Mastrevirus, Curtovirus,
Topocuvirus and Begomovirus. Among begomoviruses, one of the most important virus is TYLCV
which infects an economically important food crop i.e. tomato (Lycopersicon esculentum), all over the
world. TYLCV is also prevalent in different regions of India including HP. Five samples from
different tomato growing areas of district Solan (HP) were collected for serological detection of
TYLCV using DAS-ELISA. All samples reacted positively with the antisera. In further confirmation
studies, CP (coat protein) gene was amplified from Tohana and Manjhed isolates, using CP gene
specific primers. A 771 bp long CP sequence was amplified, sequenced and submitted to NCBI
database (Acc no-KC253231). TYLCV, the test virus was confirmed to be a begomovirus on the basis
of phylogenetic analysis while comparing it with other geminiviruses. On comparison with other
Indian tomato leaf curl viral isolates, it was found to be closely related to Dharwad isolate (GI
1160428) and not to Palampur isolate (GI 1244840), which indicates it’s origin in other than North
Indian region and evolution of a new viral strain, though it showed 70 to >90 per cent resemblance
with North Indian isolates. To predict the structure of TYLCV coat protein, ab initio approach was
followed as no structural homolog was found during database search. AAVP (Acc no-JQ 347294) was
selected to be tested as putative inhibitor of TYLCV. AAVP structure was predicted based on
similarity search with bougainvillea template. Other antimicrobial proteins; magainin, cryptdin,
moricin and Ribonuclease were also tested to check their effectiveness against TYLCV, based on
literature serach for antimicrobial agents. Ligand-receptor interaction were performed using online
server “Patchdock”. Out of five antimicrobial proteins tested, AAVP was found to be most effective
and potential viral inhibitor with highest interaction score (14156), largest active site coverage
(2455.20) with ACE value of 134.33. Based on these studies AAVP is predicted to act as a potent
inhibitor of geminiviruses.
 
Date 2016-05-31T15:30:37Z
2016-05-31T15:30:37Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66497
 
Language en
 
Format application/pdf