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MOLECULAR CHARACTERIZATION AND MANAGEMENT OF BUD NECROSIS VIRUS INFECTING TOMATO (LYCOPERSICON ESCULENTUM MILL.) IN ANDHRA PRADESH

KrishiKosh

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Title MOLECULAR CHARACTERIZATION AND MANAGEMENT OF BUD NECROSIS VIRUS INFECTING TOMATO (LYCOPERSICON ESCULENTUM MILL.) IN ANDHRA PRADESH
 
Creator RUTH, CH
 
Contributor SUBBA RAO, M
 
Subject cytokinins, auxins, vegetative propagation, biological phenomena, sugarcane, iaa, planting, regeneration, concentrates, tissue culture
MOLECULAR CHARACTERIZATION, BUD NECROSIS, TOMATO (LYCOPERSICON ESCULENTUM MILL.)
 
Description The complete nucleotide sequence was determined for coat protein (cp) gene of
Groundnut budnecrosis virus infecting tomato in Andhra Pradesh. The sequenced
region in GBNV-To isolate contained a single open reading frame of 831 bases that
could potentially code for a coat protein of 276 amino acids.
The coat protein (cp) gene sequence of GBNV -To has 93 to 99 per cent
sequence homology with that of the gene sequences of other GBNV isolates prevalent
on different hosts in India. In the present studies it is determined that the GBNV-To
isolate of tomato prevalent in A.P is regarded as the same strain of GBNV already
existing in India.
Host range studies indicated that the virus could induce different types of
symptoms on cultivated plant species viz., Arachis hypogaea, Vigna unguiculata,
Cajanus cajan, Vigna radiata, Dolichos lablab, Phaseolus vulgaris, Glycine max,
Nicotiana tabacum, Physalis minima and Capsicum annuum and weed species such as
Datura stramonium, Amaranthus viridis, Commilina bengalensis. While none of the
symptoms were observed on Parthenium hysterophorus, Pisum sativum, Helianthus
annuus, Laginaria sicenaria etc.
Insect transmission tests revealed that Thrips palmi is considered as the vector
of the virus causing bud necrosis disease in tomato and cowpea. The larvae of Thrips
palmi could acquire the virus with a minimum access period of 15 min. and the adults
only transmit the virus with 1h inoculation access period (IAP). However, optimum
virus transmission obtained with 48h of AAP in the larval stage and 48h of IAP in the
adult stage, but beyond 48h of AAP and IAP resulted in decreased virus transmission.
A single adult Thrips palmi could able to transmit the virus with a transmission
rate of 32 per cent and maximum transmission rate (100%) was achieved with 10 adults
per seedling.
In the screening trail 98 genotypes and cultivars tested under field conditions,
among the 50 NBPGR lines, 20 AICRIP genotypes, 5 IIHR genotypes and 23 cultivars
and hybrids, EC514117 and EC514190 NBPGR lines were found resistant to GBNV-
To.
Among the various combinations of treatments included in the integrated
disease management, the treatments imposed with the cultural practices such as the
crop surrounded by a strip of sorghum all around planted on July 1 st (normal planting
time) at a spacing of 60x45cm and fertilizer application at 150kg/ha with seed treatment
of Imidacloprid at 5g/kg seed followed by two sprays of Imidacloprid (0.4 ml/L)
resulted in lowest GBNV incidence.
Virus –vector population revealed that the factors dates of sowing, spacing
levels and nitrogen levels had significant influence on the occurrence of thrips
population. Lowest thrips population recorded in early planted (June 1 st ) crop, with
closer spacing (60x30cm) and lower nitrogen application (100kg/ha). Thrips
populations were low at early stage of the crop and increased progressively up to 50
DAP, and then the population decreased significantly.
Correlation studies with weather parameters on thrips population revealed that
positive correlation of thrips was recorded with maximum temperature, minimum
temperature and relative humidity and negative correlation with disease incidence,
evening relative humidity and rainfall during kharif season. The coefficient of multiple
determinations R2 values was equal to 0.953 and contribute 95.3 of variation in the
development of the disease was explained by weather parameters.
 
Date 2016-06-03T10:37:51Z
2016-06-03T10:37:51Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/66695
 
Language en
 
Relation D9025;
 
Format application/pdf
 
Publisher ACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY