MICROBIAL POPULATION DYNAMICS IN VERMICOMPOST ENRICHED WITH BIOFERTILIZERS AND THEIR EFFECT ON BLACK GRAM
KrishiKosh
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Title |
MICROBIAL POPULATION DYNAMICS IN VERMICOMPOST ENRICHED WITH BIOFERTILIZERS AND THEIR EFFECT ON BLACK GRAM
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Creator |
HARISH KUMAR, MEHTA
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Contributor |
SUBHASH REDDY, R
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Subject |
vermicomposting, bacteria, rhizobium, biofertilizers, application methods, yields, planting, vegetables, nitrogen, sowing
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Description |
The experiment was conducted during 2011-12 at college farm, Department of Agricultural Microbiology and Bioenergy, and Department of Soil Science & Agricultural Chemistry, College of Agriculture, Rajendranagar, ANGRAU, Hyderabad. Viable counts of microbes (Bacteria, Fungi, Actinomycetes, Rhizobia and Pseudomonas) and chemical properties (pH, Electrical conductivity, Organic carbon, Nitrogen, Phosphorous and Potassium content) of different vermicomposts were analysed by using standard procedures. Pure cow dung vermicompost recorded significantly higher viable counts of bacteria, fungi, actinomycetes, rhizobia and Pseudomonas and higher value of electrical conductivity, organic carbon, nitrogen, phosphorous and potassium content among all the vermicomposts, but C: N ratio recorded significantly lowest. One kg of unsterilized and one kg of sterilized commercial vermicompost were enriched using biofertilizers viz., Rhizobium, Pseudomonas @ 200 g each, separately in the standard size (30×20×5 cm) trays in eight treatments with three replications in completely randomized design. The different treatments in the experiment were T1 - Unsterilized vermicompost, T2-Unsterilized vermicompost + Rhizobium, T3.-Unsterilized vermicompost + Pseudomonas, T4- Unsterilized vermicompost +Rhizobium + Pseudomonas, T5- Sterilized vermicompost, T6- Sterilized vermicompost + Rhizobium, T7- Sterilized vermicompost + Pseudomonas, T8-Sterilized vermicompost +Rhizobium + Pseudomonas. Change in viable counts of bacteria, fungi, actinomycetes, rhizobia & Pseudomonas and available nutrient status (Nitrogen, Phosphorous and Potassium content) in the vermicompost enriched with biofertilizers , were analysed by using standard procedures at initial, 1st ,2nd ,5th and 15th day of storage period. Bacterial, fungal, actinomycetes, rhizobial and Pseudomonas population were increased slightly in the unsterilized vermicompost not enriched with biofertilizers up to 15th day of storage but not significantly. In the unsterilized and sterilized vermicompost enriched with Rhizobium and Pseudomonas biofertilizers singly as well as with combination of Rhizobium + Pseudomonas biofertilizer, the bacterial population increased significantly up to 15th day of storage. The fugal and actinomycetes population slightly increased in the unsterilized vermicompost with enrichment with Rhizobium biofertilizer up to 15th day of storage period but increase was not significant. Population of rhizobia in the unsterilized and sterilized vermicompost enriched with Rhizobium biofertilizer and Rhizobium + Pseudomonas biofertilizers increased significantly up to 15th day of storage. Population of Pseudomonas in the unsterilized and sterilized vermicompost enriched with Pseudomonas biofertilizer and Rhizobium + Pseudomonas biofertilizers increased significantly up to 15th day of storage. There was no significant increase in nitrogen, phosphorus and potassium content in the vermicompost (unsterilized and sterilized) not enriched with any biofertilizer during storage period from 0 to 15 day. In the unsterilized and sterilized vermicompost inoculated with Rhizobium + Pseudomonas showed significant increase in the nitrogen, phosphorus and phosphorus content up to 15th day of storage. In the unsterilized and sterilized vermicompost inoculated with Rhizobium and Pseudomonas separately showed significant increase in the nitrogen and phosphorus content respectively up to 15th day of storage. The field experiment was conducted following randomized block design with 11 treatments and three replications. Treatment consisted of T1-100%RDF, T2-100%RDF+ Rhizobium + Pseudomonas, T3-Vermicompost, T4-Vermicompost + Rhizobium, T5- Vermicompost + Pseudomonas, T6-Vermicompost + Rhizobium + Pseudomonas, T7- 50%RDF, T8-50%RDF+ Rhizobium + Pseudomonas, T9-50%RDF + Vermicompost + Rhizobium, T10-50%RDF + Vermicompost + Pseudomonas and T11-50%RDF + Vermicompost + Rhizobium + Pseudomonas. The growth parameters viz., plant height, root length, leaf area index, chlorophyll content, nodule no. plant-1, nodule wt. (g plant-1), dry matter accumulation (g plant-1), were recorded at different crop growth stages. Likewise observation on yield attributes viz., number of seeds pod-1, weight of 100 Seeds, seed yield and haulm yield were taken at harvest.The net return and benefit: cost ratio was calculated and presented. The protein % in seed, nutrient uptake, microbial population and available soil nitrogen, phosphorus and potassium were estimated at harvest stage. At 25 DAS and 50 DAS treatment 50 % RDF + Vermicompost + Rhizobium + Pseudomonas (T11) recorded significantly higher plant height, root length, leaf area index and spad meter value as compared to all other treatments. Treatment 50%RDF + Vermicompost + Rhizobium + Pseudomonas (T11) recorded significantly higher number of root nodule and nodule dry weight (at flowering stage), total dry matter production and seed protein content (at harvest stage) as compared to all other treatments. The combined application of 50 % RDF, vermicompost and biofertilizers were recorded highest yield contributing characters, seed and haulm yield as compared to the all other treatments and control. Significantly high nitrogen, phosphorus and potassium uptake as well as available nitrogen, phosphorus and potassium content in the soil were observed with the combined inoculation of Rhizobium + Pseudomonas along with 50% RDF and vermicompost which was significantly higher than single inoculation with Rhizobium, Pseudomonas along with 100% RDF or 50% RDF or vermicompost. At harvest stage treatment supplied with 50%RDF + Vermicompost + Rhizobium + Pseudomonas recorded significantly higher bacterial, fungal, actinomycetes, Rhizobium and Pseudomonas population as compared to the other treatments Treatment supplied with 50%RDF + Vermicompost + Rhizobium + Pseudomonas recorded highest gross return and net return but highest B: C ratio was recorded in the treatment supplied with 50%RDF + Rhizobium + Pseudomonas. The lowest net return and B: C ratio of the black gram was recorded in the treatment supplied with Vermicompost alone. |
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Date |
2016-06-16T14:26:38Z
2016-06-16T14:26:38Z 2012 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/67466
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Language |
en
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Relation |
D9126;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
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