TRANSRECTAL ULTRASONOGRAPHIC EVALUATION OF PREOVULATORY FOLLICLES IN RURAL BUFFALOES (Bubalus bubalis)
KrishiKosh
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Title |
TRANSRECTAL ULTRASONOGRAPHIC EVALUATION OF PREOVULATORY FOLLICLES IN RURAL BUFFALOES (Bubalus bubalis)
MVSc; NTRCVSc,GVRM; Acc No: D124 |
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Creator |
PRAVEEN RAJ, MUDDADA
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Contributor |
VENKATA NAIDU, G (Major)
SRINIVAS, MANDA RAGHUNATH, M ANANDA RAO, K |
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Subject |
BUFFALOES; TRANSRECTAL ULTRASONOGRAPHY; PREOVULATORY FOLLICLES;SERUM PROGESTERONE
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Description |
THESES
ABSTRACT: The present investigation was taken up to study the relationship between preovulatory follicle size, CL biometry using transrectal ultrasonography and Serum progesterone at the time of AI on the day of estrus with conception in Graded Murrah buffaloes under field conditions. A total of 70 parity buffaloes maintained under village system of rearing, free from apparent pathological abnormalities of the reproductive tract presented for the first time for AI during postpartum were utilized for this study. Once the estrus was confirmed, Body condition score (BCS) was evaluated by visual examination of the fat deposition and estrus intensity was classified based on the score card. POF size was measured ultrasonographically and grouped in to small preovulatory follicle (SPF), medium preovulatory follicle (MPF) and large preovulatory follicle (LPF). AI was performed on the day of estrus with thawed frozen semen from proven sire after checking for its post thaw motility. Ovulation was confirmed by the disappearance of POF at the same site on the following day of AI and monitored by ultrasound. The animals were re-examined using ultrasound on day 10 following insemination for determining CL size and day 30 for pregnancy diagnosis which is confirmed per rectally by day 60 post AI. Serum samples were collected on day 0 and 10 for estimation of progesterone by RIA. The mean values of BCS, intensity of estrus and serum progesterone at AI and on day 10 post AI were calculated using SPSS and the relation was analysed. Positive correlation was observed with the body condition of the buffaloes at the time of AI to POF size and intensity of estrus. Serum progesterone concentration at the time of AI and pregnancy status was negatively correlated indicating that when progesterone level drops to < 0.3 ng/ml (basal level) at the time of AI, the chances of the animal becoming pregnant was increased. The size of the POF was positively correlated to the size of the CL on day 10 in pregnant buffaloes. The overall POF diameter at the time of estrum was 12.31 ± 0.29 mm (Range 9 to 16 mm) in Graded Murrah buffaloes and 50 % of the buffaloes had POF size > 12 - ≤ 14 mm. Graded Murrah buffaloes conceived only if the POF diameter was more than 9 mm at the time of AI. However there was no significant correlation between the POF size at the time of AI and conception. Thus, the physiological maturity rather than the diameter of the follicle influenced the fertility in Graded Murrah buffaloes under field conditions. DEPARTMENT OF VETERINARY GYNAECOLOGY AND OBSTETRICS, NTR COLLEGE OF VETERINARY SCIENCE, GANNAVARAM - 521 102 SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502 (A.P) INDIA. |
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Date |
2016-12-06T17:54:48Z
2016-12-06T17:54:48Z 2014-10 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/89329
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Language |
en
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Format |
application/pdf
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Publisher |
SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502 (A.P) INDIA.
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