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TRANSRECTAL ULTRASONOGRAPHIC EVALUATION OF PREOVULATORY FOLLICLES IN RURAL BUFFALOES (Bubalus bubalis)

KrishiKosh

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Title TRANSRECTAL ULTRASONOGRAPHIC EVALUATION OF PREOVULATORY FOLLICLES IN RURAL BUFFALOES (Bubalus bubalis)
MVSc; NTRCVSc,GVRM; Acc No: D124
 
Creator PRAVEEN RAJ, MUDDADA
 
Contributor VENKATA NAIDU, G (Major)
SRINIVAS, MANDA
RAGHUNATH, M
ANANDA RAO, K
 
Subject BUFFALOES; TRANSRECTAL ULTRASONOGRAPHY; PREOVULATORY FOLLICLES;SERUM PROGESTERONE
 
Description THESES
ABSTRACT:
The present investigation was taken up to study the relationship between
preovulatory follicle size, CL biometry using transrectal ultrasonography and Serum
progesterone at the time of AI on the day of estrus with conception in Graded Murrah
buffaloes under field conditions.

A total of 70 parity buffaloes maintained under village system of rearing, free
from apparent pathological abnormalities of the reproductive tract presented for the first
time for AI during postpartum were utilized for this study. Once the estrus was
confirmed, Body condition score (BCS) was evaluated by visual examination of the fat
deposition and estrus intensity was classified based on the score card. POF size was
measured ultrasonographically and grouped in to small preovulatory follicle (SPF),
medium preovulatory follicle (MPF) and large preovulatory follicle (LPF). AI was
performed on the day of estrus with thawed frozen semen from proven sire after
checking for its post thaw motility. Ovulation was confirmed by the disappearance of
POF at the same site on the following day of AI and monitored by ultrasound. The
animals were re-examined using ultrasound on day 10 following insemination for
determining CL size and day 30 for pregnancy diagnosis which is confirmed per rectally
by day 60 post AI.

Serum samples were collected on day 0 and 10 for estimation of progesterone by
RIA. The mean values of BCS, intensity of estrus and serum progesterone at AI and on

day 10 post AI were calculated using SPSS and the relation was analysed. Positive
correlation was observed with the body condition of the buffaloes at the time of AI to
POF size and intensity of estrus. Serum progesterone concentration at the time of AI
and pregnancy status was negatively correlated indicating that when progesterone level
drops to < 0.3 ng/ml (basal level) at the time of AI, the chances of the animal becoming
pregnant was increased. The size of the POF was positively correlated to the size of the
CL on day 10 in pregnant buffaloes. The overall POF diameter at the time of estrum
was 12.31 ± 0.29 mm (Range 9 to 16 mm) in Graded Murrah buffaloes and 50 % of the
buffaloes had POF size > 12 - ≤ 14 mm. Graded Murrah buffaloes conceived only if the
POF diameter was more than 9 mm at the time of AI. However there was no significant
correlation between the POF size at the time of AI and conception. Thus, the
physiological maturity rather than the diameter of the follicle influenced the fertility in
Graded Murrah buffaloes under field conditions.
DEPARTMENT OF VETERINARY GYNAECOLOGY AND OBSTETRICS,
NTR COLLEGE OF VETERINARY SCIENCE, GANNAVARAM - 521 102
SRI VENKATESWARA VETERINARY UNIVERSITY,
TIRUPATI - 517 502 (A.P) INDIA.
 
Date 2016-12-06T17:54:48Z
2016-12-06T17:54:48Z
2014-10
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/89329
 
Language en
 
Format application/pdf
 
Publisher SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502 (A.P) INDIA.