STUDIES ON DIVERSE PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH RHIZOSPHERE OF Aconitum heterophyllum
KrishiKosh
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Title |
STUDIES ON DIVERSE PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH RHIZOSPHERE OF Aconitum heterophyllum
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Creator |
MINAKSHI
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Contributor |
SRIKOT, C.K.
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Subject |
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rhizospheric bacteria,plant growth promoting |
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Description |
Abstract It has been long recognized that many naturally occurring rhizospheric bacteria are plant growth promoting and may offer a viable substitute for chemicals. One of the important trait of PGPR is to convert insoluble forms of phosphorus to an accessible form for increasing plant growth. Little is known about the composition of phosphate solubilizing bacterial diversity associated with rhizosphere of medicinal plant. Therefore, the present study was aimed to explore the diversity of P-solubilizing bacterial communities colonizing the rhizosphere soil and endosphere of medicinal plant Aconitum heterophyllum grown in natural conditions of trans Himalayan region of Himachal Pradesh. A total of one hundred and seventy one rhizospheric and endorhizospheric bacteria were isolated from soil and root samples of Aconitum heterophyllum collected from two different sites of Kangra and Lahaul valley and were screened for phosphate solubilization, siderophore production and nitrogen fixation. A total of one hundred and ten P-solubilizing bacterial isolates were selected for quantitative estimation of Psolubilzation, and twenty highest P-solubilizing bacterial isolates were selected and further screened for multifarious plant growth promoting traits. Out of twenty P-solubilizing bacterial isolates ten isolates were selected on the basis of high PGP traits for growth promotion of tomato seedlings under net house conditions for sixty days. Treatment with isolate Ka(1)5r showed maximum per cent increase in various growth parameters of tomato seedlings and therefore, was found to be most efficient plant growth promoting rhizobacteria which was further selected for optimization of P-solubilization and IAA production. Optimized cultural conditions resulted in maximum P-solubilization (197 μg/ml) after 96 h of incubation at 35°C in PVK broth (having concentration of TCP 1%) of pH 6.0 with 1% inoculum size of culture. In case of optimized cultural conditions for IAA production, the maximum IAA production (42 μg/ml) was observed after 96h of incubation at 35°C in LB broth (with 1.0% of tryptophan concentration) of pH 8.0 ammended with an inoculum size of 2%. The most efficient bacterial isolate Ka(1)5r was identified as Bacillus subtilis by 16S rRNA sequencing. Thus, the ability of performing multifarious plant growth promoting activities in tandem suggest PSB isolates for plant growth promotion and has significance to develop a cost-effective ecofriendly multifunctional biofertilizer. |
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Date |
2016-12-19T15:03:57Z
2016-12-19T15:03:57Z 2015 |
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Type |
Thesis
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Identifier |
48384
http://krishikosh.egranth.ac.in/handle/1/91081 |
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Language |
en
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Format |
application/pdf
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