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STUDIES ON DIVERSE PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH RHIZOSPHERE OF Aconitum heterophyllum

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Title STUDIES ON DIVERSE PLANT GROWTH PROMOTING RHIZOBACTERIA ASSOCIATED WITH RHIZOSPHERE OF Aconitum heterophyllum
 
Creator MINAKSHI
 
Contributor SRIKOT, C.K.
 
Subject ---
rhizospheric bacteria,plant growth promoting
 
Description Abstract
It has been long recognized that many naturally occurring rhizospheric bacteria are plant growth
promoting and may offer a viable substitute for chemicals. One of the important trait of PGPR is to
convert insoluble forms of phosphorus to an accessible form for increasing plant growth. Little is known
about the composition of phosphate solubilizing bacterial diversity associated with rhizosphere of
medicinal plant. Therefore, the present study was aimed to explore the diversity of P-solubilizing bacterial
communities colonizing the rhizosphere soil and endosphere of medicinal plant Aconitum heterophyllum
grown in natural conditions of trans Himalayan region of Himachal Pradesh. A total of one hundred and
seventy one rhizospheric and endorhizospheric bacteria were isolated from soil and root samples of
Aconitum heterophyllum collected from two different sites of Kangra and Lahaul valley and were
screened for phosphate solubilization, siderophore production and nitrogen fixation. A total of one
hundred and ten P-solubilizing bacterial isolates were selected for quantitative estimation of Psolubilzation,
and twenty highest P-solubilizing bacterial isolates were selected and further screened for
multifarious plant growth promoting traits. Out of twenty P-solubilizing bacterial isolates ten isolates
were selected on the basis of high PGP traits for growth promotion of tomato seedlings under net house
conditions for sixty days. Treatment with isolate Ka(1)5r showed maximum per cent increase in various
growth parameters of tomato seedlings and therefore, was found to be most efficient plant growth
promoting rhizobacteria which was further selected for optimization of P-solubilization and IAA
production. Optimized cultural conditions resulted in maximum P-solubilization (197 μg/ml) after 96 h of
incubation at 35°C in PVK broth (having concentration of TCP 1%) of pH 6.0 with 1% inoculum size of
culture. In case of optimized cultural conditions for IAA production, the maximum IAA production (42
μg/ml) was observed after 96h of incubation at 35°C in LB broth (with 1.0% of tryptophan
concentration) of pH 8.0 ammended with an inoculum size of 2%. The most efficient bacterial isolate
Ka(1)5r was identified as Bacillus subtilis by 16S rRNA sequencing. Thus, the ability of performing
multifarious plant growth promoting activities in tandem suggest PSB isolates for plant growth
promotion and has significance to develop a cost-effective ecofriendly multifunctional
biofertilizer.
 
Date 2016-12-19T15:03:57Z
2016-12-19T15:03:57Z
2015
 
Type Thesis
 
Identifier 48384
http://krishikosh.egranth.ac.in/handle/1/91081
 
Language en
 
Format application/pdf