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Molecular characterization of begomovirus causing leaf curl disease in sunflower

KrishiKosh

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Title Molecular characterization of begomovirus causing leaf curl disease in sunflower
 
Creator L. S, Vanitha
 
Contributor K. T, Rangaswamy
 
Subject diseases, dna, vegetables, biological phenomena, planting, pcr, viruses, genes, genetic structures, nucleotides
 
Description Sunflower (Helianthus annuus L.) is one of the three important
edible oilseed crops grown in the world after soybean and groundnut.
The crop has been found suffering from many diseases. Recently a leaf
curl disease caused by a begomovirus has been observed in Northern
Karnataka, India. Sunflower leaf curl virus infected plant are associated
with symptoms such as vein clearing, vein thickening, upward curling of
leaves, reduction in the leaf size, enations, stunted growth with reduced
ear head resulted poor seed set. The occurance of disease incidence up to
(58.20%) in MARS, Pamanakallur (10%) of Raichur district. Murdi (10%),
Narasapur (25%), Kanakagiri (12.50%), Somanakoppa (16%), Mangaluru
(25%), Beur (15.50%), Gundlanur (8%), Betagere (10.50%), Katarki (20%)
disease incidence was noticed in villages of Koppal District, Northren
Karnataka.
Twenty viruliferous whiteflies were required for 100 per cent
transmission of SuLCV. Among twelve plant species tested, SuLCV was
successfully infected the plant species viz, tobacco, tomato, Zinnia,
Parthenium and Acanthospermum sp. The virus was detected using CP
specific primers which amplified ~570 bp product of core CP of the viral
genome which was cloned and sequenced. The sequences shared highest
coat protein nucleotide identity (97.5%) with ToLCKV-(Luc) and ToLCBV-
(Ban2) isolates. The complete nucleotide sequence of DNA A component
was determined to be 2761 nucleotides. Full genome (DNA-A) Sequence
of SuLCV showed maximum identity with ToLCKV and ToLCV-[Ban-II]
isolates.
SuLCV beta DNA satellite molecule of ~1.3 kb was amplified and
sequence consisted of 1373 nt. Maximum nucleotide sequence identity of
(94.07%) was with Potato apical leaf curl disease associated satellite DNA
beta. The alpha DNA satellite molecule was amplified and the sequence
consisted of 1350 nt showed maximum nucleotide sequence identity of
(94%) with Tobacco curly shoot alpha DNA satellite molecule associated
with leaf curl disease on wild sunflower.
 
Date 2016-05-19T10:57:23Z
2016-05-19T10:57:23Z
2012-11-27
 
Type Thesis
 
Identifier Th-10429
http://krishikosh.egranth.ac.in/handle/1/66112
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru