Study on Association of Growth Hormone Gene Polymorphism with Milk Production in Cattle
KrishiKosh
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Title |
Study on Association of Growth Hormone Gene Polymorphism with Milk Production in Cattle
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Creator |
Punya, J.
Jayashankar, M.R. Girish Kumar, V. Shrikrishna Isloor |
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Contributor |
Nagaraja, C.S.
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Subject |
Study on Association of Growth Hormone Gene Polymorphism with Milk Production in Cattle
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Description |
PG. Thesis
The genetic variation at the growth hormone gene in a HF cross bred population and to study the possible association of the identified polymorphism with first lactation milk yield. Two fragments of bovine growth hormone gene covering part of intron 3 and exon 5 were PCR amplified from 50 HF cross bred cattle and subjected to RFLP with MspI and AluI enzymes respectively. PCR-RFLP of exon 5 of growth hormone gene with AluI yielded monomorphic pattern of 223, 171 and 52 bp fragments. The sequencing of PCR product confirmed the pattern as heterozygous (LV). No homozygotes were observed in the population. The PCR-RFLP of intron 3 region of growth hormone gene with MspI yielded three genotypes. The frequency of genotypes Msp (+/+), Msp (+/-) and Msp (-/-) were 0.28, 0.12 and 0.60 respectively. The population was not in Hardy Weinberg Equilibrium with respect to Msp polymorphism. The average first lactation milk yield of HF cross bred cows with genotypes Msp (+/+), Msp (+/-) and Msp (-/-) were 2493 kgs, 2473 kgs and 2558 kgs respectively and were not significantly different indicating that the SNP had no influence on the first lactation milk yield in the population studied. |
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Date |
2016-11-14T15:36:38Z
2016-11-14T15:36:38Z 2012-12-27 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/85448
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Language |
en
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Format |
application/pdf
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Publisher |
Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar
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