Evaluation of genetic purity and seed enhancement treatments in cotton hybrids and parental lines
KrishiKosh
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Title |
Evaluation of genetic purity and seed enhancement treatments in cotton hybrids and parental lines
Ph.D. |
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Creator |
MENKA
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Contributor |
Yadav, Shiv K.
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Subject |
sowing, hybrids, cotton, germinability, storage, developmental stages, land resources, seed treatment, planting, spacing
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Description |
In the recent years there has been phenomenal increase in the area and production of cotton hybrids in India. With the development of new hybrids, there is need for more effective technology to ensure the highest seed quality including genetic purity of these hybrids, as the genetic improvements are delivered through seeds at an increased cost to the growers. Keeping this in view, studies on genetic purity testing and seed enhancement treatments on cotton hybrids was carried out in the Division of Seed Science and Technology, IARI, New Delhi-12. To test the genetic purity of cotton hybrids namely, dch-32 rahb 87 and their parental lines fourty ssr markers were screened from the cotton marker database. Out of these, eight primers namely, BNL 169, BNL 2590, BNL 2895, BNL 840, BNL 3103, BNL 2544, BNL 2572 and BNL 3441 were polymorphic among their parental lines. These highly informative primers not only differentiated the two hybrids but also confirmed the parentage of the f1 hybrid. Thus, these ssr primers can be used to quick and reliable testing of genetic purity of these two popular interspecific cotton hybrids. The initial seed quality assessment in Bt Express and non Bt Express hybrid for various vigour parameters showedthatnon Bt Express hybrid had higher vigour owing to higher seedling length and dry weight. However, there was no significant difference in the germination percent of the two hybrids. Before storage, the seeds treated with cruiser (94.0%) and azotobacter+microphos (93.5%) exhibited higher germination over all other treatments.Though all seeds quality enhancement treatments maintained seed germination above the minimum seed certification standard (65.0%), the seeds treated with azotobacter+ microphos @ 10g/kg of seed recorded highest first count (79.0%), germination (88.6%), seedling dry weight (0.236g), vigour index I (2651) and vigour index II (21.0) up to 12 months of storage. In the field, seeds treated with azotobacter + microphos (10g each/kg of seed) and thiram+ imidacloprid (2.0 g/kg seed+7.5ml/kg seed) proved to be best treatment in terms of higher field emergence, final plant stand establishment, early initiation of flowering, 50% flowering and 50% ball opening. Spacing of 75×60cm was found to be significantly superior over 75×75cm and 75×30cmas it resulted in earlier initiation of flowering, 50% flowering, 50% ball opening as well as increase in field emergence and yield per plant. |
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Date |
2016-03-10T20:08:58Z
2016-03-10T20:08:58Z 2013 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/65064
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Language |
en
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Format |
application/pdf
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Publisher |
IARI,DIVISION OF SEED SCIENCE AND TECHNOLOGY, NEW DELHI
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