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Evaluation of genetic purity and seed enhancement treatments in cotton hybrids and parental lines

KrishiKosh

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Title Evaluation of genetic purity and seed enhancement treatments in cotton hybrids and parental lines
Ph.D.
 
Creator MENKA
 
Contributor Yadav, Shiv K.
 
Subject sowing, hybrids, cotton, germinability, storage, developmental stages, land resources, seed treatment, planting, spacing
 
Description In the recent years there has been phenomenal increase in the area and production of
cotton hybrids in India. With the development of new hybrids, there is need for more
effective technology to ensure the highest seed quality including genetic purity of these
hybrids, as the genetic improvements are delivered through seeds at an increased cost
to the growers. Keeping this in view, studies on genetic purity testing and seed
enhancement treatments on cotton hybrids was carried out in the Division of Seed
Science and Technology, IARI, New Delhi-12. To test the genetic purity of cotton
hybrids namely, dch-32 rahb 87 and their parental lines fourty ssr markers were
screened from the cotton marker database. Out of these, eight primers namely, BNL
169, BNL 2590, BNL 2895, BNL 840, BNL 3103, BNL 2544, BNL 2572 and BNL
3441 were polymorphic among their parental lines. These highly informative primers
not only differentiated the two hybrids but also confirmed the parentage of the f1
hybrid. Thus, these ssr primers can be used to quick and reliable testing of genetic
purity of these two popular interspecific cotton hybrids.
The initial seed quality assessment in Bt Express and non Bt Express hybrid for
various vigour parameters showedthatnon Bt Express hybrid had higher vigour owing
to higher seedling length and dry weight. However, there was no significant difference
in the germination percent of the two hybrids. Before storage, the seeds treated with
cruiser (94.0%) and azotobacter+microphos (93.5%) exhibited higher germination over
all other treatments.Though all seeds quality enhancement treatments maintained seed
germination above the minimum seed certification standard (65.0%), the seeds treated
with azotobacter+ microphos @ 10g/kg of seed recorded highest first count (79.0%),
germination (88.6%), seedling dry weight (0.236g), vigour index I (2651) and vigour
index II (21.0) up to 12 months of storage.
In the field, seeds treated with azotobacter + microphos (10g each/kg of seed)
and thiram+ imidacloprid (2.0 g/kg seed+7.5ml/kg seed) proved to be best treatment in
terms of higher field emergence, final plant stand establishment, early initiation of
flowering, 50% flowering and 50% ball opening. Spacing of 75×60cm was found to be
significantly superior over 75×75cm and 75×30cmas it resulted in earlier initiation of
flowering, 50% flowering, 50% ball opening as well as increase in field emergence and
yield per plant.
 
Date 2016-03-10T20:08:58Z
2016-03-10T20:08:58Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/65064
 
Language en
 
Format application/pdf
 
Publisher IARI,DIVISION OF SEED SCIENCE AND TECHNOLOGY, NEW DELHI