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STUDY ON POPULATION DYNAMICS AND EFFECT OF PESTICIDES ON Pseudomonas fluorescens And Trichoderma harzianum USED AS ANTAGONISTS AGAINST BACTERIAL LEAF BLIGHT OF RICE IN IPM SYSTEM

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Title STUDY ON POPULATION DYNAMICS AND EFFECT OF PESTICIDES ON Pseudomonas fluorescens And Trichoderma harzianum USED AS ANTAGONISTS AGAINST BACTERIAL LEAF BLIGHT OF RICE IN IPM SYSTEM
Ph.D.
 
Creator OM PRAKASH
 
Contributor Pratibha Sharma
 
Subject ##Unable to generate tags d7510.pdf
 
Description T-8779
The growth and survival ability of Trichoderma harzianum (Th3) and Pseudomonas fluorescens (RRb11) was evaluated on different substrates as FYM, Peat moss, soil (single & their combinations) under protected condition without rhizosphere. Their survivability also observed in inoculants and sterilized double distilled water at fifteen days interval. The bioagent inoculants were mixed with sterilized substrates (@10 g/kg) and double distilled water (10 g/l). Inoculated plastic pots were kept in protected condition (polyhouse) at 28±2˚C and R.H. >80 %. Appropriate moisture in substrates was maintained regularly by spraying tap water. Amongst the substrates and their combinations, soil+FYM (1:1) supported maximum growth (CFU) of both fungal and bacterial antagonists viz. 78.33×104 g-1 and 128.67×105 g-1 respectively at 15 days after inoculation (DAI). The similar trend of population increased/or declined was observed for both antagonists. Population size of P. fluorescens in talc based bioformulation (inoculants) was high initially (12.3 ×108 g-1) and subsequently declined significantly. The temporal population dynamics of Trichoderma harzianum and Pseudomonas fluorescens was evaluated in rice rhizosphere under protected conditions after single and combined application of antagonists inoculants . The population of T. harzianum and P. fluorescens reached maximum at 42 days after sowing viz. 11.17×104 g-1 soil and 7.48×106 g-1 soil, respectively and thereafter, declined. In combined application, generally lesser population was observed than single treatment. A booster dose of antagonists also applied at the time of transplanting (21 DAS) through root dipping in the aqueous solution prepared by mixing 10g inoculants in one litre distilled water for two hours. In phyllosphere, population observed after foliar spray at weekly interval for a month. Both antagonists population increased slightly in initial 15 days of sowing and then declined significantly. In field experiments, population of fungal and bacterial antagonists reached its pinnacle at 60 DAS as observed over two growing period.
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Population of bioagents was significantly higher in treated plot that of check (Untreated). In another experiment, the spatial population dynamics evaluated at differentiated depth levels (1,5,10 and 15 cm) in rice rhizosphere under open field conditions. It was observed that maximum population of antagonists exist near soil line (1-5 cm) and below 5cm depth, spore/cell counts declined significantly.
 
Date 2016-08-22T10:49:48Z
2016-08-22T10:49:48Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/73243
 
Language en_US
 
Format application/pdf
 
Publisher IARI, DIVISION OF PL ANT PATHOLOGY