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MOLECULAR CHARACTERISATION OF ISOLATES OF Phytophthora infestans (Mont.) de Bary CAUSING LATE BLIGHT DISEASE IN POTATO AND TOMATO

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Title MOLECULAR CHARACTERISATION OF ISOLATES OF Phytophthora infestans (Mont.) de Bary CAUSING LATE BLIGHT DISEASE IN POTATO AND TOMATO
 
Creator SAPTARSHI, GHOSH
 
Contributor RANGASWAMY, K. T
 
Subject potatoes, diseases, vegetables, fungi, planting, beverages, phytophthora infestans, biological phenomena, pcr, genotypes
 
Description Potato and Tomato are important vegetable crops of India and Karnataka, suffer
due to many diseases. The oomycete fungus Phytophthora infestans (Mont.) de Bary, the
causal agent of late blight of potato and tomato is one of the most devastating microbial
pathogens worldwide.
Two isolate of P. infestans from tomato each from Kolar and GKVK and one
isolate from potato from Hassan were characterized for mitochondrial haplotypes, mating
types and metalaxyl sensitivity. The two isolates from tomato and the one from potato
were found to be of Ia mitochondrial haplotype. This suggested a change in population
and migration of P. infestans. The isolates were found to be of A2 mating type according
to the restriction digestion profiles obtained. The potato isolate from Hassan and the
tomato isolate from GKVK were found to be insensitive to metalaxyl. The tomato isolate
from Kolar was found to be moderately sensitive to metalaxyl.
In vitro efficacy of new fungicide molecules like metalaxyl, Cymoxanil +
Mancozeb, Fenamidone + Mancozeb, Famoxadone + Mancozeb, Famoxadone +
Cymoxanil and Trifloxystrobin were tested against the isolates of P. infestans at
concentration of 10, 25, 50, 100 and 200 ppm. The study reflected high effectiveness of
Cymoxanil+ Mancozeb, Trifloxystrobin and Fenamidone + Mancozeb in inhibition of the
pathogen. Trifloxystrobin was the most effective against P. infestans followed by
Cymoxanil + Mancozeb, Fenamidone + Mancozeb. Whereas, metalaxyl was found to be
least effective even at 200 pp concentration.
The effect of date of planting on late blight severity on potato was studied at ARS
Madenur, Hassan. Planting of Potato in early June gave 4 times higher yield. Whereas,
the late plantings suffered heavy damage in the early stages of the crop growth with high
disease severity and yield was significantly reduced. Meteorological data was collected
and analyzed which reflected that the incidence and severity of the disease increased with
frequent rainfall along with low night temperatures.
 
Date 2016-12-22T10:31:40Z
2016-12-22T10:31:40Z
2011-11-18
 
Type Thesis
 
Identifier Th-9909
http://krishikosh.egranth.ac.in/handle/1/92049
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences GKVK, Bangalore