Record Details

FUNCTIONAL VALIDATION OF ROOT GROWTH ASSOCIATED GENES BY OVEREXPRESSION IN MODEL PLANTS FOR ABIOTIC STRESS TOLERANCE.

KrishiKosh

View Archive Info
 
 
Field Value
 
Title FUNCTIONAL VALIDATION OF ROOT GROWTH ASSOCIATED GENES BY OVEREXPRESSION IN MODEL PLANTS FOR ABIOTIC STRESS TOLERANCE.
 
Creator NETHRA, P
 
Contributor NADARADJAN, S
 
Description In India, abiotic stress especially drought is the major limitations for sustained crop
productivity. Plants have different adaptive strategies such as cellular tolerance, water
mining, water conservation and WUE are considered to be important mechanisms for drought
tolerance. Although water mining is an important character, a little effort has been made to
exploit root growth associated characters. Therefore, to understand the relevance of root traits
under water stress condition, attempts have been made to overexpress a few genes associated
with root growth and development such as Alfin1, CDPK1 (Calcium dependent protein
kinase) and OsRAA1(Oryza sativa root architecture associated 1) in model plant tobacco
(Nicotiana tabacum). Over expression cassette were developed using an invitro
transformation protocol to generate transgenic tobacco plants. The integration and expression
of root genes in putative transgenic was confirmed by RT-PCR and Southern blot analysis
respectively. The T0 lines were evaluated and a few selected lines were advanced to the next
generation for further analysis. The transgenic lines expressing OsCDPK1, OsRAA1 and
OsAlfin1 exhibited better resistance to NaCl (300mM) and PEG (-8 bar) stress compared to
wild type plants. Under drought condition, almost all the transgenic plants showed improved
tolerance as evidenced by relative water content, membrane integrity, chlorophyll stability,
cell viability and osmotic adjustment. Also the transgenic lines showed better resistance to
ethylene induced senescence as indicated by membrane damage and chlorophyll estimation.
Hence the data suggest that overexpression of the root related genes can improve cellular
tolerance besides increasing the root growth.
 
Date 2016-12-16T10:54:22Z
2016-12-16T10:54:22Z
2011-03-26
 
Type Thesis
 
Identifier TH-10137
http://krishikosh.egranth.ac.in/handle/1/90479
 
Language en
 
Format application/pdf