FUNCTIONAL VALIDATION OF ROOT GROWTH ASSOCIATED GENES BY OVEREXPRESSION IN MODEL PLANTS FOR ABIOTIC STRESS TOLERANCE.
KrishiKosh
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Title |
FUNCTIONAL VALIDATION OF ROOT GROWTH ASSOCIATED GENES BY OVEREXPRESSION IN MODEL PLANTS FOR ABIOTIC STRESS TOLERANCE.
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Creator |
NETHRA, P
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Contributor |
NADARADJAN, S
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Description |
In India, abiotic stress especially drought is the major limitations for sustained crop productivity. Plants have different adaptive strategies such as cellular tolerance, water mining, water conservation and WUE are considered to be important mechanisms for drought tolerance. Although water mining is an important character, a little effort has been made to exploit root growth associated characters. Therefore, to understand the relevance of root traits under water stress condition, attempts have been made to overexpress a few genes associated with root growth and development such as Alfin1, CDPK1 (Calcium dependent protein kinase) and OsRAA1(Oryza sativa root architecture associated 1) in model plant tobacco (Nicotiana tabacum). Over expression cassette were developed using an invitro transformation protocol to generate transgenic tobacco plants. The integration and expression of root genes in putative transgenic was confirmed by RT-PCR and Southern blot analysis respectively. The T0 lines were evaluated and a few selected lines were advanced to the next generation for further analysis. The transgenic lines expressing OsCDPK1, OsRAA1 and OsAlfin1 exhibited better resistance to NaCl (300mM) and PEG (-8 bar) stress compared to wild type plants. Under drought condition, almost all the transgenic plants showed improved tolerance as evidenced by relative water content, membrane integrity, chlorophyll stability, cell viability and osmotic adjustment. Also the transgenic lines showed better resistance to ethylene induced senescence as indicated by membrane damage and chlorophyll estimation. Hence the data suggest that overexpression of the root related genes can improve cellular tolerance besides increasing the root growth. |
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Date |
2016-12-16T10:54:22Z
2016-12-16T10:54:22Z 2011-03-26 |
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Type |
Thesis
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Identifier |
TH-10137
http://krishikosh.egranth.ac.in/handle/1/90479 |
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Language |
en
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Format |
application/pdf
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