Chemical induction of male sterility and histological studies in okra (Abelmoschus esculentus (L.) Monech.)
KrishiKosh
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Title |
Chemical induction of male sterility and histological studies in okra (Abelmoschus esculentus (L.) Monech.)
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Creator |
K.D.Deepak
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Contributor |
V.K.Deshpande
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Subject |
Seed Science and Technology
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Description |
A field and laboratory experiments were conducted at Main Agricultural Research Station, UAS, Dharwad during kharif 2006 to understand the effect of gametocides on induction of male sterility and histological events accompanying with induced male sterility in okra. The study included three chemicals at three concentrations sprayed at three stages. Degree of pollen sterility varied with the nature of chemical, concentration, stage of spray and number of sprays. MH @ 200 ppm sprayed at 20+30+40 days after sowing (DAS) resulted in highest pollen sterility (84.33%) followed by ethrel @ 1250 ppm (82.10%) and GA3 @ 400 ppm (82.00%) sprayed at same stages. Pollen sterility increased with increasing concentration and number of sprays. The plant height increased significantly due to GA3 and decreased with ethrel and MH. All chemicals decreased the leaf area and increased the number of branches significantly. However, number of branches decreased as concentration increased. Number of days to initiation of flowering and 50 per cent flowering decreased significantly due to GA3 over control and increased with MH and ethrel. Higher concentration of all gametocides resulted in reduction in number of fruits per plant, fruit set (%), number of seeds per pod and seed yield per plant by causing ovular sterility. The effective dose (200 ppm at 20+30+40 DAS) of MH caused 8.87 per cent ovular sterility whereas ethrel (1250 ppm) shown highest ovular sterility (14.83%) at same number of sprays. Germination per cent and 100 seed weight found to be non-significant. Histological studies revealed that shriveling of anther, anther locule, microspores, degeneration of anther wall, clumping of meiocytes and early degeneration of tapetum in MH induced pollen sterility whereas all these features were normal in control plants. |
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Date |
2016-10-25T17:05:49Z
2016-10-25T17:05:49Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/81966
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Format |
application/pdf
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Publisher |
UAS, Dharwad
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