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Chemical induction of male sterility and histological studies in okra (Abelmoschus esculentus (L.) Monech.)

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Title Chemical induction of male sterility and histological studies in okra (Abelmoschus esculentus (L.) Monech.)
 
Creator K.D.Deepak
 
Contributor V.K.Deshpande
 
Subject Seed Science and Technology
 
Description A field and laboratory experiments were conducted at Main Agricultural Research
Station, UAS, Dharwad during kharif 2006 to understand the effect of gametocides on
induction of male sterility and histological events accompanying with induced male sterility
in okra.
The study included three chemicals at three concentrations sprayed at three stages.
Degree of pollen sterility varied with the nature of chemical, concentration, stage of spray
and number of sprays. MH @ 200 ppm sprayed at 20+30+40 days after sowing (DAS)
resulted in highest pollen sterility (84.33%) followed by ethrel @ 1250 ppm (82.10%) and
GA3 @ 400 ppm (82.00%) sprayed at same stages. Pollen sterility increased with increasing
concentration and number of sprays.
The plant height increased significantly due to GA3 and decreased with ethrel and
MH. All chemicals decreased the leaf area and increased the number of branches
significantly. However, number of branches decreased as concentration increased. Number of
days to initiation of flowering and 50 per cent flowering decreased significantly due to GA3
over control and increased with MH and ethrel.
Higher concentration of all gametocides resulted in reduction in number of fruits per
plant, fruit set (%), number of seeds per pod and seed yield per plant by causing ovular
sterility. The effective dose (200 ppm at 20+30+40 DAS) of MH caused 8.87 per cent ovular
sterility whereas ethrel (1250 ppm) shown highest ovular sterility (14.83%) at same number
of sprays. Germination per cent and 100 seed weight found to be non-significant.
Histological studies revealed that shriveling of anther, anther locule, microspores,
degeneration of anther wall, clumping of meiocytes and early degeneration of tapetum in MH
induced pollen sterility whereas all these features were normal in control plants.
 
Date 2016-10-25T17:05:49Z
2016-10-25T17:05:49Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/81966
 
Format application/pdf
 
Publisher UAS, Dharwad