Delignification and Bioethanol production from Agroresidues
KrishiKosh
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Title |
Delignification and Bioethanol production from Agroresidues
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Creator |
Raghavendra B.Havannavar
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Contributor |
Geeta Shrinalli
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Subject |
Agricultural Microbiology
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Description |
The agricultural crop residues such as paddy straw, wheat straw and sugarcane bagasse are abundantly available having rich source of sugars. Ethanol can be blended directly in petrol, upto 20%. Presently, 5% of ethanol is being blended along with diesel for transportation in few states in India. This indicates the demand for ethanol. In the present study, pre-treatment process was developed for hydrolyzing paddy straw, wheat straw and sugarcane bagasse to get maximum fermentable sugars and further conversion to ethanol by fermentation process. The substrates were powdered into two different particle size 1 cm and less than 5 mm. The powdered substrates were pre-treated with 1.5% and 2% sulphuric acid and alkali (sodium hydroxide) at the concentrations of 2% and 3%. Among these, the substrates with particle size less than 5 mm and pre-treated with 3% alkali has given maximum cellulase content in sugarcane bagasse 0.695 g/g followed by wheat straw 0.593 g/g and paddy straw 0.560 g/g. The maximum hemicellulase content was observed in paddy straw 0.132 g/g followed by sugarcane bagasse 0.123 g/g and wheat straw 0.117 g/g. The delignified substrates (with 3% NaOH) were subjected for microbiological and commercial cellulase enzyme pre-treatment for further saccharification. Among fungal cultures Trichoderma reesei has yielded maximum reducing sugar in all the substrates. The commercial cellulase enzyme yielded maximum reducing sugar as compared to that of fungi. The hydrolysate thus obtained was used for screening of yeast cultures. Among these, the substrates pre-treated with commercial cellulase enzyme were recorded maximum ethanol compared to the substrates pre-treated with Trichoderma reesei. Among yeast strains Pachysolen tannophilus NCIM 3445 has showed comparatively higher ethanol yield in all the substrates. The chemical delignification process developed followed by the cellulase enzyme treatment yields maximum reducing sugars and yeast Pachysolen tannophilus was identified suitable for ethanol production. |
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Date |
2016-10-17T10:51:17Z
2016-10-17T10:51:17Z 2006 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/80661
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Format |
application/pdf
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Publisher |
UAS, Dharwad
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