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Influence of different additives to increase shelf life of rhizobial inoculants for mungbean (Vigna radiata L.)

KrishiKosh

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Title Influence of different additives to increase shelf life of rhizobial inoculants for mungbean (Vigna radiata L.)
 
Creator Sehrawat, Anju
 
Contributor Suneja, Sunita
 
Subject Mungbean crop (Vigna radiata L.), Rhizobium sp. strains MB703 & MB1503, yeast extract mannitol broth, additives, glycerol, poly vinyl pyrrolidone (PVP), gum arabic (GA)
 
Description In the present study, attempts were made to enhance the survival/shelf life of liquid rhizobial
inoculants (Rhizobium sp. strains MB703 & MB1503) through addition of additives such as glycerol,
poly vinyl pyrrolidone (PVP) and gum arabic (GA). In all the inoculants, viable count of Rhizobium sp.
strains MB703 & MB1503 decreased with increase in storage time. All the inoculants prepared in
amended media showed higher viable count as compared to inoculant prepared in yeast extract
mannitol broth (YEMB) at 180 d of storage. Maximum viable count at 180 d was observed in inoculant
prepared in YEMB amended with 2% PVP for both the strains. All the inoculants prepared with
additives showed higher viable count as compared to inoculant without additive at 180 d of storage.
Maximum viable count at 180 d was observed in inoculant prepared in YEMB amended with 2% GA
for both the strains. Survival of strain MB1503 was slightly better as compared to strain MB703 at 180
d of storage. To evaluate the efficacy of liquid rhizobial inoculants for mungbean crop, an experiment
in pots was conducted.Nodule weight, shoot weight and total plant nitrogen were better in inoculated
treatments as compared to uninoculated control. Response of fresh inoculant and 90 days old inoculant
of strain MB703 was almost same with respect to above plant growth parameters whereas response of
fresh inoculant was slightly better than 90 d old inoculant of MB1503. Some of the treatments
inoculated with 90 d old inoculant + amendments (added before or after the growth) were significantly
better with respect to nodule weight, shoot weight and total plant nitrogen than treatment inoculated
with 90 d old inoculant. Among all the inoculated treatments, treatment inoculated with 90 d old
inoculant amended with 1% PVP (added after the growth) showed maximum nodule weight, shoot
weight and total plant nitrogen for both the strains. Response of strain MB703 with respect to all
growth parameters was better as compared to strain MB1503.
 
Date 2016-09-12T09:56:52Z
2016-09-12T09:56:52Z
2013
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76386
 
Language en
 
Format application/pdf
 
Publisher CCSHAU