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Isolation and characterization of chickpea endophytic bacteria and their effect on nodulation, yield, nutrient uptake and soil properties in urdbean and chickpea

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Title Isolation and characterization of chickpea endophytic bacteria and their effect on nodulation, yield, nutrient uptake and soil properties in urdbean and chickpea
 
Creator Rana, Monika
 
Contributor Ramesh Chandra
 
Subject Isolation, characterization, chickpeas, Rhizobium, soil biology, nodulation, nutrient uptake, soil properties, black gram, chickpeas
 
Description Thesis-PhD
Bacteria that live inside the plant tissues without causing any damage or change in their hosts are called endophytic bacteria. Thirty-three endophytic bacterial strains were isolated from the roots and nodules of chickpea grown at N.E.B. C.R.C. of the University. The isolates were characterized on the basis of cultural, biochemical and plant growth promoting (PGP) traits. Most of the isolates were Gram negative and showed multiple PGP activity, i.e., indole acetic acid production, siderophore production, and phosphate solubilization. The 10 selected isolates namely, NE2, NE3, NE5, NE10, NE12, NE14, NE16, NE17, R4 and R5 these were identified by 16S rRNA gene sequence analysis. Performance of these 10 different endophytic bacteria was evaluated in pot and field experiments during Kharif and Rabi seasons of 2013 and 2013- 14 in a Sandy loam soil at Pantnagar in terms of root nodulation, growth and yields of urdbean and chickpea and soil biological properties Treatments consisted of soil inoculation with Rhizobium sp. in urdbean and Mesorhizobium sp. in chickpea alone and with 10 endophytes and an uninoculated control. Rhizobium sp. or Mesorhizobium sp. alone showed marginal benefits in nodulation, plant dry matter production, grain and straw yields, N and P uptake and soil properties. Combined use of Rhizobium sp./ Mesorhizobium sp. with different endophytic bacteria further increased the nodule number, by 3.4 to 15.6 % and 4.7 to 81.8%, nodule dry weight, by 4.6 to 35.9 % and 2.3 to 69.3 %, and plant dry weight, by 1.1 to 41.6 % and 2.4 to 66.5% of urdbean and chickpea, respectively, over Rhizobium sp /Mesorhizobium sp. Alone inoculation at different crop age. Endophytic bacteria NE10 recorded significantly more grain yield urdbean and chickpea of 16.8 and 18.4% and straw yield of 4.9 and 26.1% over Rhizobium sp./Mesorhizobium sp. alone. The co-inoculated endophytic bacteria improved N uptake by urdbean and chickpea grain from 11.3 to 59.5 % and 16.1 to 44.3 %, N uptake by straw from 5.4 and 36.9% and 3.3 to 43.5%, P uptake by grain from 1.8 to 32.7% and 10.5 to 39.7 % and P uptake by straw from 4.7 to 35.4% and 5.8 to 66.9 % over Rhizobium sp./ Mesorhizobium sp. alone, respectively. Their dual inoculation in urdbean and chickpeaincreased the SMBC from 14.8 to 97.7 and 11.4 to 83.4% and 14.1 to 109.8 % and 14.2 to 88.7% and dehydrogenase activity from 4.8 to 39.3 and 2.8 to 17.3% and 2.0 to 39.0 % and 2.8 to 19.3% over Rhizobium sp. /Mesorhizobium sp. alone at 45 DAS and at harvest of crop, respectively. The co-inoculated endophytic bacteria also favoured acid and alkaline phosphatise activities at both the intervals in both the crops. NE 10 (Bacillus cereus) with Rhizobium sp. or Mesorhizobium sp. was found most efficient in improving the nodulation, plant dry matter, yield, nutrient uptake and soil properties in urdbean and chickpea.
 
Date 2016-10-26T11:40:22Z
2016-10-26T11:40:22Z
2014-07
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82086
 
Language en
 
Format application/pdf
 
Publisher G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)