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GENETIC ANALYSIS OF DROUGHT TOLERANCE IN CROSSES OF TWO ROW AND SIX ROW BARLEY (Hordeum vulgare L.)

KrishiKosh

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Title GENETIC ANALYSIS OF DROUGHT TOLERANCE IN CROSSES OF TWO ROW AND SIX ROW BARLEY (Hordeum vulgare L.)
 
Creator Bornare Satish Santosh
 
Contributor Prof. L. C. Prasad
 
Subject Barley, drought tolerence, gene action, combining ability
 
Description Ph. D.
The present investigation entitled “Genetic analysis of drought tolerance in crosses of two row and six row barley (Hordeum vulgare L.)” was conducted at the Agriculture Research Farm, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi. Out of several genotypes grown and maintained by All India Co-ordinated Barley Improvement Project in a crossing block at the Institute Agricultural Farm at three dates of sowing, ten diverse genotypes (4 testers and 6 lines) were selected based on phenotypic and molecular diversity. Four testers (six-rowed) were crossed with six lines (two-rowed) to produced 24 F1s’ (excluding reciprocals) in line x tester fashion, using tester as female during rabi season, 2010-11. The experimental materials (24 F1s’ along with parents including standard check i. e. K- 603) were
grown under rainfed condition in a single row plot of 5 m length in the Compact Family Randomized Block Design with three replications during rabi Season, 2011-12. Standard agronomic practices were followed to raise a good crop.
Per se performance of parents and crosses, based on randomly selected ten plants from each replication, were assessed for various yield and drought related traits viz., days to 50% flowering, days to maturity, plant height, number of effective tillers, spike length, awn length, number of grains per spike, thousand grain weight, harvest index, grain yield per plant, chlorophyll content, leaf rolling, stay green, stomatal conductance and proline content. These sampled F1 plants were selfed to produce F2 seeds. Simultaneously fresh F1s’ and back crosses (B1 and B2) were also made. Six generations P1, P2, F1, F2, B1 and B2 of the crosses made/selected on the basis of contrasting parents for each traits were grown in Compact Family Randomized Block Design with three replications under two environments i.e. moisture stress (rainfed) and moisture non- stress (irrigated) conditions during rabi Season, 2012-13 to study
different types of gene action and their interaction through generation mean analysis and also for isolating drought tolerant plants in F2 generation. Data on randomly sampled 10 competitive plants from each of the parents and F1s’, 20 plants from backcrosses (B1 and B2) and 50 plants from each F2 population from each replication were recorded on the above said characters.
The parents BCU 4922, BCU 4910, BCU 4932 Lakhan and Karan 16 might have exhibited some genetic mechanism to thrive well on larger areas and to escape from initial and terminal heat through manipulations of various yield components. To synthesize a dynamic population with most of the favourable genes accumulated, it will be pertinent to make use of the aforesaid parents which were good general combiners for several characters, in multiple crossing programmes.
Therefore, in barley breeding programme, aiming to improve the yield and drought tolerance, it might be suggested that, crosses involving these parents may be expected to yield transgressive segregants in segregating generations. Apart from conventional breeding methods relying solely upon additive or additive x additive type of gene action, population improvement appears to be a promising alternative. Test weight, awn length, stomatal conductance, chlorophyll content and proline content might be taken as selection criteria for isolation of segregants under moisture stress condition. The crosses, such as, BCU 4910 x K 603 and BCU 4922 x K 603 were yielded promising transgressive segregants under both environmental conditions henceforth the further fixation of genes of segregants derived from these by continued selfing and molecular validation with known drought tolerant/resistant markers might be done. The barley varieties BCU 4922, BCU 4910 and Karan 16 and K 603 should be given due consideration while improving grain yield along with drought/resistant genotypes.
 
Date 2016-09-20T22:30:47Z
2016-09-20T22:30:47Z
2015
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/77840
 
Language en
 
Relation P0033;
 
Format application/pdf
 
Publisher Institute of Agricultural Sciences, Banaras Hindu University