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EVALUATION OF CHITOSAN-POVIDONE IODINE COMPOSITE FILMS ON CUTANEOUS WOUND HEALING IN RABBITS

KrishiKosh

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Title EVALUATION OF CHITOSAN-POVIDONE IODINE COMPOSITE FILMS ON CUTANEOUS WOUND HEALING IN RABBITS
MVSC;CVSc;TPTY; Acc No:T1458
 
Creator SUMIRAN, N
 
Contributor VEENA, P (Major)
SURESH KUMAR, R.V
SRILATHA, Ch
 
Subject CUTANEOUS WOUND; RABBITS ; CHITOSAN; COMPOSITE FILMS; CHITOSAN-POVIDONE IODINE
 
Description THESES
ABSTRACT:
Eighteen rabbits were divided into three groups of six each to study the efficacy of chitosan and chitosan-povidone iodine composite films on cutaneous wounds of 1x1 cm area. The wounds in all the groups were protected with external bandaging after thorough cleaning with normal saline to prevent infection. Chitosan films were applied in group II and chitosan – povidone iodine composite films in group III . The efficacy of these biocasings were studied based on gross, physiological, haematological, biochemical and histopathological changes at different periods of observations.
Wounds were evaluated clinically by observing the appearance, extent of cicatrisation and percentage of wound contraction in all the groups of animals at different time intervals. Early wound healing was observed in group II (15.33±0.21 days) as compared to group I (18.5±0.22) and group III (16.5±0.22). None of the animals showed host rejection or local tissue reaction. Accumlation of wound fluid was more in group I as compared to other two groups. The biocasings were clearly adhered to the wound surface and prevented haemorrhage.
Physiological parameters like temperature, respiration and pulse rates did not show any significant changes during the period of observations and were within the physiological range.
Total erythrocyte count, total leucocyte count, haemoglobin, PCV, neutrophil count, and lymphocyte count showed significant differences. However monocyte count, eosinophil count and basophil count didnot show any significant changes.
The wound healing was evaluated microscopically and by biochemical estimation of hydroxyproline , hexosamine and trace elements like zinc, iron, copper in biopsy specimens collected from wound at 7th, 14th day after treatment. The values of hydroxyproline and all the elements were lower in the initial stages of wound healing, whereas hexosamine values decreased with the progression of healing. None of the animals in all the groups showed CRP positive reaction.
Histopathologically, early epithelialisation and marked fibrovascular proliferation were noticed in chitosan treated animals when compared to other groups. . However metaplastic changes and gaint cells were not noticed, suggesting safety of biocasings.
Based on the above findings, it was concluded that use of chitosan film for cutaneous wound healing in rabbits was effective without any adverse effects.
DEPARTMENT OF VETERINARY SURGERY AND RADIOLOGY,
COLLEGE OF VETERINARY SCIENCE ,
SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517 502
 
Date 2017-01-03T15:24:24Z
2017-01-03T15:24:24Z
2012-10
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/94212
 
Language en
 
Format application/pdf
 
Publisher Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P