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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF ELITE RICE VARIETIES FOR TOLERANCE TO PRE-HARVEST SPROUTING

KrishiKosh

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Title MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF ELITE RICE VARIETIES FOR TOLERANCE TO PRE-HARVEST SPROUTING
 
Creator MANJEGOWDA, B B
 
Contributor KESHAVULU, K
 
Subject MORPHOLOGICAL, MOLECULAR, CHARACTERIZATION, ELITE RICE VARIETIES, TOLERANCE, PRE-HARVEST, SPROUTING
 
Description The present investigation was undertaken to assess the pre-harvest sprouting
trait in rice varieties by employing morphological trait and SSR markers. The material
for investigation comprised of 25 rice varieties. The field study was carried out at
experimental farm, ICAR, Indian Institute of Rice Research, ICAR, Rajendranagar,
Hyderabad, during Kharif 2015-16 and the laboratory study was carried out at the
Department of Seed Science and Technology, College of Agriculture, Professor
Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad.
The studies of morphological assessment were carried out by the pre-harvest
sprouting test method. This test revealed the resistance and susceptibility of the
varieties to the pre-harvest sprouting. The varieties JGL 17004, WGL 13400, JGL
11727, JGL 3844, JGL 18047, WGL 3962, MTU 1010, MTU 1001, RNR 2354, RNR
15048 and RNR 15038 were found to be highly resistant to pre-harvest sprouting.
While, the varieties WGL 347, WGL 283, JGL 1798, WGL 536, WGL 44, JGL 20175,
JGL 384, WGL 32100, WGL 14, RNR 18833, WGL 20471, RNR 2458, BPT 5204 and
RNR 10754 were found to be highly susceptible to PHS.
Molecular characterization revealed that, out of 118 genomic SSR markers
which are distributed across rice genome analysed for molecular diversity, 36 were
found to be polymorphic with average allele frequency of 3 per marker with average
polymorphic information content (PIC) value of 0.58. The markers RM26213, RM4477
and RM18414 found to be highly polymorphic.
Cluster analysis showed that genetic relatedness of 25 rice varieties collections
ranged from 0.51 to 0.84 i.e., 51-84 %. At an arbitrary cut-off at 51 percent similarity
level on a dendrogram, the whole collections were clustered into two major clusters
viz., cluster I (24 varieties) and cluster II (RNR 15048). The major cluster ‘I’ is divided
in two sub clusters i.e., cluster I A contains two varieties viz., MTU 1010 and MTU
1001 and cluster I B (contains 22 genotypes) at 54 % of similarity. Cluster I B was
further divided into two sub clusters i.e., Cluster I B1 containing 13 varieties viz., JGL
17004, WGL 13400, WGL 536, WGL 283, WGL 347, JGL 1798, JGL 3844, WGL
32100, JGL 11727, WGL 44, JGL 384, JGL 20175 and WGL 20471 and the cluster I
B2 contains 9 varieties viz., RNR 2354, WGL 3962, WGL 14, RNR 18833, RNR
10754, JGL 18047, RNR 2458, BPT 5204 and RNR 15038 at 58% genetic similarity.
The varieties MTU 1010 and MTU 1001 were observed to be part of the same
cluster indicating that grouping may be in accordance with the morphological trait i.e.
pre- harvest sprouting. Interestingly RNR 15048 was also found to be part of the same
cluster where MTU 1010 and MTU 1001 were present and all the three were noted to
be highly resistant to pre-harvest sprouting. This might be due to one of the parents of
RNR 15048 was MTU 1010. This indicates that clustering pattern observed in the
present study is in concordance with the pedigree of the genotypes analyzed and also
could be due to the fact that all the three varieties were also highly resistant to PHS.
The remaining genotypes were grouped in different sub clusters.
The marker RM26972 is found to be specific for PHS tolerant variety RNR
2354 and the marker RM26213 is found to be specific for PHS tolerant varieties JGL
17004 and MTU 1010. So we could use these markers in gene mapping and eventually
for the application of marker assisted selection (MAS) for the trait pre-harvest sprouting
tolerance.
In the present study, the morphological assessment clearly showed the
resistance and susceptibility of the rice varieties for pre-harvest sprouting. That could
use for developing new rice varieties tolerant to pre-harvest sprouting. The
polymorphism information content value clearly demonstrated that the markers used in
this study were highly informative. The information about genetic diversity of these
varieties might be very useful for proper selection of parents and rice breeding
programmes and also for gene mapping and marker assisted selection (
 
Date 2017-01-02T15:17:02Z
2017-01-02T15:17:02Z
2016
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/93995
 
Language en
 
Relation D10006;
 
Format application/pdf
 
Publisher PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY RAJENDRANAGAR, HYDERABAD