Genetic Variabilityin calpain and calpastatin Loci and Association of calpastatin Polimorphism with Carcass and Meat Quality Traits in Bandur Sheep
KrishiKosh
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Title |
Genetic Variabilityin calpain and calpastatin Loci and Association of calpastatin Polimorphism with Carcass and Meat Quality Traits in Bandur Sheep
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Creator |
NavinKumar S.
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Contributor |
M.R. Jayashankar
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Subject |
meat, livestock, animal products, sexual reproduction, genotypes, genes, acidity, alleles, pcr, productivity
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Description |
The present study was conducted to investigate the genetic variability in calpain and calpastatin loci, to characterize carcass and meat quality traits and to determine association of calpastatin gene polymorphism with carcass and meat quality traits in 100 Bandur ram lambs distributed over core area of the home tract as well as from LRIC (S), KVAFSU, Nagamangala. A 620 bp of CAST (exon1C/1D) and 192 bp of CAPN (exons 5 and 6) genes were amplified by PCR using published primers. The PCR-RFLP analysis of CAST gene revealed frequencies of 0.24, 0.59 and 0.17 for genotype MM, MN and NN and 0.535 and 0.465 for allele M and N, respectively. The Chi square test indicated that the studied population was in Hardy-Weinberg equilibrium. Five SNPs were identified in intron between exon 1C and 1D of CAST. The PCR-SSCP analysis of CAPN gene revealed genotype frequency of 0.672 and 0.295 for AA and AB and allele frequency of 0.820 and 0.180 for A and B, respectively. Among the carcass and meat quality traits studied, high loin eye area measurement was recorded indicating higher muscular development in Bandur ram lambs. No significant difference was observed between carcass traits and CAST genotypes. The NN genotype had significantly lower Warner-Bratzler Shear Force value and significantly higher total ash percentage than MM and MN genotypes. The present study suggests the CAST gene as a possible candidate gene for meat tenderness in Bandur sheep. |
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Date |
2016-06-17T09:40:41Z
2016-06-17T09:40:41Z 2015-05-01 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/67492
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Language |
en
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Format |
application/pdf
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Publisher |
Karanataka Veterinary, Animal and Fisheries Sciences University, Bidar
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