“STUDIES ON PRODUCTION AND BIOCHEMICAL COMPOSITION OF GANODERMA spp. (REISHI MUSHROOM) UNDER CHHATTISGARH CONDITIONS”
KrishiKosh
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Title |
“STUDIES ON PRODUCTION AND BIOCHEMICAL COMPOSITION OF GANODERMA spp. (REISHI MUSHROOM) UNDER CHHATTISGARH CONDITIONS”
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Creator |
Sharma, Deepti
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Contributor |
Thakur, Dr. M.P.
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Subject |
PRODUCTION, BIOCHEMICAL COMPOSITION, GANODERMA spp. (REISHI MUSHROOM), CHHATTISGARH
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Description |
The present study entitled “Studies on production and biochemical composition of Ganoderma spp. (Reishi mushroom) under Chhattisgarh conditions” was conducted in Mushroom Research Laboratory at the Department of Plant Pathology, Department of Aromatic and Medicinal Plants, Department of Soil Science and Department of Biotechnology, IGKV, Raipur. An effort was made to systematically understand the growth, yield and changes in biochemical constituents of the substrate during cultivation and biochemical constituent present in basidiocarp and mycelium of Ganoderma spp. to derive comprehensive information which may help in standardization of production technology of Reishi mushroom under Chhattisgarh condition. Fifteen Ganoderma spp. were collected during the survey and these were characterized morphologically, out of these five were brought to pure culture and used for further studies. Two isolates out of six studied were found to be different from rest of four isolates. Among media, MEA + linseed extract, MEA +Dextrose (1%), MEA + Dextrose (1.5%) and MEA + Dextrose (2%) showed excellent diametric growth of Ganoderma spp. However, among five strains, the growth of local strain of Ganoderma (GR/L/04/1) was very fast. Higher biomass was obtained was obtained in MEA + Sunflower extract liquid broth followed by G. applanatum, G. lucidum (IS) and local strain of Ganoderma (GR/L/04/1). Fruiting body in local strain of Ganoderma was also formed within one month after inoculation. Temperature of 30-35C was optimum for the maximum radial growth of Ganoderma spp. Bajra grains were found to be most suitable for spawn development as it took significantly less time. The other substrates like wheat grains, sorghum grains and sugarcane baggase also gave good results. All the strains showed fruiting induction at different times but it was earlier in bajra grains in case of local strain of Ganoderma (GR/L/04/1). In both the year 2004 and 2005, higher yield was obtained in wheat straw + saw dust substrate. Fruiting body was also obtained in sugarcane baggase which was not reported earlier. The mycelial colonization was very fast when sugarcane bagasse and sunflower straw were supplemented with 15% wheat bran. Mycelial extract of local strain of Ganoderma was found to completely inhibit the growth of Coprinus spp. and Sclerotium rolfsii but partially suppressed the growth of Trichoderma viride and Rhizoctonia solani. The growth of these contaminants was also found to be suppressed when fruit body extract was used at higher concentration. Degradation of cellulose, lignin and total sugars was less after 20 days of inoculation but it increased rapidly when fungus reached to fruiting stage. No particular trend was found in phenol content as it showed marked variation at different stages. Nutritionally, the mycelium and fruit body of G. lucidum was found to be more superior than G. applanatum with respect to protein, moisture, fat, carbohydrates and mineral content. |
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Date |
2016-02-06T11:17:42Z
2016-02-06T11:17:42Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/64182
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Language |
en
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Format |
application/pdf
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