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Utilization of pearl millet for ethanol production

KrishiKosh

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Title Utilization of pearl millet for ethanol production
 
Creator Poonia, Bijender
 
Contributor Leela Wati
 
Subject Pearl millet, Ethanol production, Fermentation, Molasses, Saccharomyces cerevisiae, Hydrolysate, Peptone
 
Description Ethanol is a known feed stock for countless chemicals
and potable purposes. Recently the use of ethanol for fuel
purpose is catching up faster world over. It is produced by
fermentation of molasses by Saccharomyces cerevisiae in Indian
distilleries. Molasses has become more expensive due to its
decontrol and deteriorated in quality. Therefore, an alternate
substrate that would be both cheaper and readily available in
quantities, need to be sought and examined. Pearl millet grains
were considered as potential substrate for ethanol production
because of high starch content (56.9%).
xv
The optimum conc. of pearl millet flour slurry for
liquefaction by a-amylase was found to be 30% which was
liquefied by 600ml of enzyme at 85ºC in 10 min under shaking
conditions (140 rpm). Saccharification of liquefied starch of pearl
millet hybrids by spirizyme (750ml) at 60ºC under shaking
conditions resulted in production of maximum 15.9% (w/v)
reducing sugars in 2h. Fermentation of hydrolysate of various
pearl millet cultivars at 30±2ºC by S. cerevisiae at pitching rate of
0.5% (w/v) generated 10.6-11.4% (v/v) ethanol with in 36 hrs.
under stationary conditions. No significant effect of
supplementation of hydrolysate with urea, peptone and yeast
extract was observed for ethanol production suggesting that the
pearl millet flour has sufficient N and P required for ethanolic
fermentation.
 
Date 2016-11-22T13:53:26Z
2016-11-22T13:53:26Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/87000
 
Language en
 
Format application/pdf
 
Publisher CCSHAU