Utilization of pearl millet for ethanol production
KrishiKosh
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Title |
Utilization of pearl millet for ethanol production
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Creator |
Poonia, Bijender
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Contributor |
Leela Wati
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Subject |
Pearl millet, Ethanol production, Fermentation, Molasses, Saccharomyces cerevisiae, Hydrolysate, Peptone
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Description |
Ethanol is a known feed stock for countless chemicals and potable purposes. Recently the use of ethanol for fuel purpose is catching up faster world over. It is produced by fermentation of molasses by Saccharomyces cerevisiae in Indian distilleries. Molasses has become more expensive due to its decontrol and deteriorated in quality. Therefore, an alternate substrate that would be both cheaper and readily available in quantities, need to be sought and examined. Pearl millet grains were considered as potential substrate for ethanol production because of high starch content (56.9%). xv The optimum conc. of pearl millet flour slurry for liquefaction by a-amylase was found to be 30% which was liquefied by 600ml of enzyme at 85ºC in 10 min under shaking conditions (140 rpm). Saccharification of liquefied starch of pearl millet hybrids by spirizyme (750ml) at 60ºC under shaking conditions resulted in production of maximum 15.9% (w/v) reducing sugars in 2h. Fermentation of hydrolysate of various pearl millet cultivars at 30±2ºC by S. cerevisiae at pitching rate of 0.5% (w/v) generated 10.6-11.4% (v/v) ethanol with in 36 hrs. under stationary conditions. No significant effect of supplementation of hydrolysate with urea, peptone and yeast extract was observed for ethanol production suggesting that the pearl millet flour has sufficient N and P required for ethanolic fermentation. |
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Date |
2016-11-22T13:53:26Z
2016-11-22T13:53:26Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/87000
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Language |
en
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Format |
application/pdf
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Publisher |
CCSHAU
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