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Identification of resistant source(s) and DNA markers linked to genomic regions conferring dry root rot resistance in chickpea (Cicer arietinum L.)

KrishiKosh

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Title Identification of resistant source(s) and DNA markers linked to genomic regions conferring dry root rot resistance in chickpea (Cicer arietinum L.)
 
Creator Talekar, Sidramappa
 
Contributor K. P, Viswanatha
 
Subject ---
 
Description An investigation was carried out in chickpea at AICRP on
chickpea, University of Agricultural Sciences, GKVK, Bangalore, to
screen a set of 520 genotypes under laboratory condition to identify
resistant source for dry root rot disease, unravel the genetics of disease
resistance and identify DNA markers linked to genomic regions
conferring dry root rot resistance. Among 520 genotypes, three lines
viz., PG 06102, BG 2094 and IC 552137 were identified as resistant for
dry root rot that could be used as potential donors for development of
resistant varieties. F3 segregating population derived from the cross L
550 (susceptible) PG 06102 (resistant) was screened under
laboratory condition against dry root rot disease to understand the
genetics of resistance. The segregation pattern indicated monogenic
inheritance for dry root rot with resistance being dominant over
susceptibility. Fifty two out of 381 SSR primers used for parental
polymorphism differentiated the two parents (L 550 and PG 06102). F2
resistant and susceptible bulks were genotyped using polymorphic
SSR primers. Only four of the 52 SSR primers could differentiate the
resistant and susceptible bulks. Linkage analysis with 129 F2
individuals using SSR markers from bulk segregant analysis of the
cross L 550 × PG 06102 revealed that two viz., ICCM 0299 and ICCM
0120b were co-segregating with resistance to dry root rot as evidenced
by significant mean sum of squares due to between marker classes.
The characters days to 50 per cent flowering (25.93 %) and seed yield
per plant (24.73 %) together contributed fifty per cent to the total
diversity. Three resistant genotypes PG 06102, BG 2094 and IC
552137 were grouped separately into different clusters indicating
enormous diversity between three lines and their potential use in
breeding programmes to develop high yielding and dry root rot
resistant genotypes in chickpea.
 
Date 2016-05-23T13:50:09Z
2016-05-23T13:50:09Z
2013-11-07
 
Type Thesis
 
Identifier Th-10687
http://krishikosh.egranth.ac.in/handle/1/66222
 
Language en
 
Format application/pdf
 
Publisher University of Agricultural Sciences, Bengaluru