SUPEROXIDE DISMUTASE AND ASCORBATE PEROXIDASE GENE EXPRESSION ANALYSIS IN TOMATO UNDER MOISTURE STRESS CONDITIONS
KrishiKosh
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Title |
SUPEROXIDE DISMUTASE AND ASCORBATE PEROXIDASE GENE EXPRESSION ANALYSIS IN TOMATO UNDER MOISTURE STRESS CONDITIONS
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Creator |
Jamwal, Deeksha
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Contributor |
Rai, Gyanendra Kumar
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Subject |
ROS, DROUGHT, MDA, APX, SOD
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Description |
M.Sc. Thesis
TOMATO (Solanum lycopersicum), a major horticultural crop consumed all over the world, suffers heavy losses due to drought. Periods of little or no rainfall can lead to a metrological condition called drought. Water deficit causes various physiological and biochemical effects on plant populations. In response, plants utilize a number of protective mechanisms to maintain normal cellular metabolism and prevent damage to cellular components. Tolerance to water stress in plants is generally associated with maintenance of plant water status. There has been need to explore the status of Reactive Oxygen Species (ROS) production in plants and their contribution to antioxidant defence system. Two genotypes Kashi Vishesh (Solanum lycopersicum Mill.) and WIR-13706 (Lycopersicum ceresiforme) were collected from IIVR, Varanasi. Each genotype was sown in ten pots under both controlled and drought conditions. Estimation of ROS was done by measuring MDA activity. MDA activity was found to increase in two cultivars grown under drought stress conditions and ranged between 9.24 – 13.7757 (nmol g–1fw) and 3.6166 - 5.6933 (nmol g–1 fw) under stress and normal conditions respectively. The cultivars which were allowed to grow under controlled conditions showed significant increase in the relative water content. The relative water content ranged between 186.3813 %- 561.7227% under controlled conditions and 134.4007% - 355.7383% under drought stress condition. Electrolyte Leakage conductivity increases under controlled conditions. The per cent conductivity ranged between 87.57% - 102.3667% under controlled conditions and between 69.41% -87.7066% under drought conditions. The ascorbate peroxidase activity increased under drought stress condition. Under controlled conditions, ascorbate peroxidase activity ranged between 12.9133 – 16.1883 (min–1 g–1 fw) and 14.3 - 19.5 (min–1 g–1 fw) under drought stress condition. Super-Oxide dismutase activity ranged between 10.225 - 14.01 (min–1 g–1 fw) under controlled conditions and 17.1546 - 26.165 min–1 g–1 fw) under drought stress condition. SOD gene expression increased from 1.905 to 2.995 in WIR-13706 and 1.375 to 3.38 in Kashi Vishesh under drought conditions. APX gene expression increases from 0.788424 to 1.962221 in WIR-13706 and 0.982878 to 1.842013 in Kashi Vishesh under drought conditions. Since, drought resistant variety (WIR-13706) showed more expression than the susceptible species (Kashi Vishesh), hence this wild variety can be used for further crop improvement programmes. |
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Date |
2016-09-29T11:07:38Z
2016-09-29T11:07:38Z 2016 |
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Type |
Thesis
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Identifier |
J-14-MB-20
http://krishikosh.egranth.ac.in/handle/1/79421 |
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Language |
en
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Format |
application/pdf
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Publisher |
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu
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