ESTIMATION OF GENETIC DIVERSITY AND CROSSABILITY PATTERN IN Grewia optiva Drummond
KrishiKosh
View Archive InfoField | Value | |
Title |
ESTIMATION OF GENETIC DIVERSITY AND CROSSABILITY PATTERN IN Grewia optiva Drummond
|
|
Creator |
VERMA, ARCHANA
|
|
Contributor |
SINGH, N.B.
|
|
Subject |
genotypes, genetics, polymorphism, enzymes, developmental stages, crossing over, rapd, germinability, locus, fruits
Grewia optiva Drummond ,genetic diversity,crossability |
|
Description |
455 ABSTRACT The present investigation entitled “Estimation of genetic diversity and crossability pattern in Grewia optiva Drummond” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S. Parmar University of Horticulture & Forestry Nauni, Solan (HP) during 2009-2011. Twenty five RAPD and eighteen ISSR primers were effective in revealing polymorphisms among different genotypes of Grewia optiva. RAPD exhibited 96.31 per cent polymorphism among thirty genotypes, out of the total, 163 scorable bands, 157 showed polymorphism and six bands exhibited monomorphism. Total numbers of amplified and polymorphic fragments generated per ISSR primer revealed 91.72 per cent polymorphism among genotypes. Genotype SO-12 (Kasauli) came as outliner as revealed by both RAPD and ISSR study. Nine enzyme systems were studied for genetic diversity studies, out of which viz, polymorphic loci identified were CAT Ia, EST Ia, EST IIa, PER Ia, PER Ib, PER IIa, PER IIb, MDH Ia, MDH Ib, MDH IIa, MDH IIIa, MDH IIIb, SOD IIIa and SOD IIIb, showing considerable and very useful variation among the thirty genotypes of Grewia optiva. Out of 13 loci, 9 loci were polymorphic and 4 monomorphic were in nature. Crossability pattern studies revealed that genotypes under study were cross compatible should be involved in intra-specific breeding programme. In all the twenty seven successful crosses between different genotypes were obtained. Per cent successful cross was highest in cross HA-4 (Hamirpur Kanal) × SI-10 (Seenaghat) that was 70 per cent. The data for various nursery traits viz., collar diameter, plant height and number of leaves for various crosses was recorded when seedlings were 8 months old. Collar diameter for various crosses ranged between 2.22 mm to 4.43 mm. maximum value for collar diameter was recorded for cross CH-6 (Saru) × SI-11 (Adgu) i.e. 4.43 mm. The maximum plant height was 25.8 cm which was recorded for cross SI-10 (Seenaghat) × SO-3 (Dharja). The maximum number of leaves was found in the cross SI-10 (Seenaghat) × SO-3 (Dharja). Self-incompatibility studies in the selected genotypes showed that genotypes were self-compatible showed seed set and varying degree of selfing percentage. Pollen grains of different genotypes were collected in bulk from April to June by direct extraction from flowers. Maximum pollen size (74.37 μ) was in genotype SI-10 (Seenaghat) and minimum in HA-4 (Hamirpur Kanal) (57.16 μ). Germination percentage was maximum (20.67 per cent) in genotype BI-4 (Kuthira). For freshly collected pollen genotype HA-4 (Hamirpur Kanal) showed highest percentage i.e. 82 per cent. For the pollen stored for one year at -20°C genotype SI-10 (Seenaghat) showed maximum viability percentage i.e. 74.50 and minimum for genotype SO-2 (Nauni) i.e. 59.17 per cent. Formation of pollen tube occurred between 14 to 20 hours after pollination and loss of receptivity became evident by brown discoloration |
|
Date |
2016-06-17T14:47:55Z
2016-06-17T14:47:55Z 2012 |
|
Type |
Thesis
|
|
Identifier |
http://krishikosh.egranth.ac.in/handle/1/67549
|
|
Language |
en
|
|
Format |
application/pdf
|
|