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ESTIMATION OF GENETIC DIVERSITY AND CROSSABILITY PATTERN IN Grewia optiva Drummond

KrishiKosh

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Title ESTIMATION OF GENETIC DIVERSITY AND CROSSABILITY PATTERN IN Grewia optiva Drummond
 
Creator VERMA, ARCHANA
 
Contributor SINGH, N.B.
 
Subject genotypes, genetics, polymorphism, enzymes, developmental stages, crossing over, rapd, germinability, locus, fruits
Grewia optiva Drummond ,genetic diversity,crossability
 
Description 455
ABSTRACT
The present investigation entitled “Estimation of genetic diversity and crossability pattern in Grewia
optiva Drummond” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S.
Parmar University of Horticulture & Forestry Nauni, Solan (HP) during 2009-2011. Twenty five RAPD and
eighteen ISSR primers were effective in revealing polymorphisms among different genotypes of Grewia optiva.
RAPD exhibited 96.31 per cent polymorphism among thirty genotypes, out of the total, 163 scorable bands, 157
showed polymorphism and six bands exhibited monomorphism. Total numbers of amplified and polymorphic
fragments generated per ISSR primer revealed 91.72 per cent polymorphism among genotypes. Genotype SO-12
(Kasauli) came as outliner as revealed by both RAPD and ISSR study. Nine enzyme systems were studied for
genetic diversity studies, out of which viz, polymorphic loci identified were CAT Ia, EST Ia, EST IIa, PER Ia,
PER Ib, PER IIa, PER IIb, MDH Ia, MDH Ib, MDH IIa, MDH IIIa, MDH IIIb, SOD IIIa and SOD IIIb,
showing considerable and very useful variation among the thirty genotypes of Grewia optiva. Out of 13 loci, 9
loci were polymorphic and 4 monomorphic were in nature. Crossability pattern studies revealed that genotypes
under study were cross compatible should be involved in intra-specific breeding programme. In all the twenty
seven successful crosses between different genotypes were obtained. Per cent successful cross was highest in
cross HA-4 (Hamirpur Kanal) × SI-10 (Seenaghat) that was 70 per cent. The data for various nursery traits viz.,
collar diameter, plant height and number of leaves for various crosses was recorded when seedlings were 8
months old. Collar diameter for various crosses ranged between 2.22 mm to 4.43 mm. maximum value for collar
diameter was recorded for cross CH-6 (Saru) × SI-11 (Adgu) i.e. 4.43 mm. The maximum plant height was 25.8
cm which was recorded for cross SI-10 (Seenaghat) × SO-3 (Dharja). The maximum number of leaves was
found in the cross SI-10 (Seenaghat) × SO-3 (Dharja). Self-incompatibility studies in the selected genotypes
showed that genotypes were self-compatible showed seed set and varying degree of selfing percentage. Pollen
grains of different genotypes were collected in bulk from April to June by direct extraction from flowers.
Maximum pollen size (74.37 μ) was in genotype SI-10 (Seenaghat) and minimum in HA-4 (Hamirpur Kanal)
(57.16 μ). Germination percentage was maximum (20.67 per cent) in genotype BI-4 (Kuthira). For freshly
collected pollen genotype HA-4 (Hamirpur Kanal) showed highest percentage i.e. 82 per cent. For the pollen
stored for one year at -20°C genotype SI-10 (Seenaghat) showed maximum viability percentage i.e. 74.50 and
minimum for genotype SO-2 (Nauni) i.e. 59.17 per cent. Formation of pollen tube occurred between 14 to 20
hours after pollination and loss of receptivity became evident by brown discoloration
 
Date 2016-06-17T14:47:55Z
2016-06-17T14:47:55Z
2012
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/67549
 
Language en
 
Format application/pdf