MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF COTTON [Gossypium hirsutum (L.)] HYBRIDS AND THEIR PARENTAL LINES
KrishiKosh
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Title |
MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF COTTON [Gossypium hirsutum (L.)] HYBRIDS AND THEIR PARENTAL LINES
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Creator |
ARUNA, P
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Contributor |
SAMBASIVA RAO, P
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Subject |
planting, genetic processes, regeneration, pigeonpeas, genes, vegetative propagation, bacteria, biological development, cytokinins, biological phenomena
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Description |
The present investigation was undertaken to characterize the cotton hybrids and their parents using morphological and molecular markers. A field experiment was conducted at college farm with randomized block design and laboratory studies were carried out at Institute of Biotechnology, College of Agriculture, ANGRAU, Rajendranagar, Hyderabad. A set of 21 qualitative and quantitative morphological traits and 16 Simple Sequence Repeats (SSRs) markers were used for the analysis. The study was also intended to develop molecular ID fingerprints using SSR markers. The leaf characters viz., leaf size, leaf shape, leaf colour, leaf incision, leaf hairiness at peak flowering stage under field conditions were recorded. The leaf shape, size and pubescence were useful in differentiating the cotton genotypes. Morphological characters like growth habit, stem pigmentation, stem hairiness, plant height were recorded. The growth habit and pubescence on stem could be easily identified and categorized in to different groups. The flowering character viz., days to 50 per cent flowering, petal colour, petal spot, anther colour, stigma position, boll shape, bract type and number of serrations on bract were useful in categorizing and differentiation of the cotton genotypes. The quantitative characters like plant height, days to 50 percent flowering and days to maturity were also useful for characterization of cotton genotypes as they exhibited variation. DNA finger printing studies were useful to determine the hybridity and genetic purity testing of cotton hybrids. About 16 most informative microsatellite markers were employed for fingerprinting six cotton hybrids and their parental lines. These highly informative primers not only differentiated the parent genotypes but also confirmed the parentage of their true hybrids. Out of 16 primers only five microsatellite markers differentiated the six hybrids and their parental lines. The SSR banding profiles of each of the hybrid and their parents were observed manually for the presence or absence of distinct bands. Comparison of the distinct banding patterns of the parents and their progenies determined the true hybrids. The microsatellite marker, BNL 3449 amplified alleles specific to differentiate parental lines of NSPHH 5 likewise BNL 3255 primer for NDLHH 240 and WGHH 2, BNL 1317 primer for CSH 198, BNL 3090 primer for CICR 2 and JESPR 148 primer for LAHH 4 . Thus the microsatellite banding pattern of parents compared with its respective hybrids was clearly able to recognize the true hybrid and the fingerprinting data were analyzed to create ‘Molecular IDs’ needed for genotype characterization which was more informative and useful in identifying genotypes. |
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Date |
2016-06-17T14:12:08Z
2016-06-17T14:12:08Z 2012 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/67536
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Language |
en
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Relation |
D9127;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
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