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Development and molecular marker analysis of F1 and/or backcross plants from crosses between aerobic and high yielding rice cultivars

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Title Development and molecular marker analysis of F1 and/or backcross plants from crosses between aerobic and high yielding rice cultivars
 
Creator Jogender Singh
 
Contributor Jain, R.K.
 
Subject Rice, Irrigation, Pplanting, Genotypes, Vegetative propagation, Yields, Crossing over, Genetics, Sowing, Weather hazards
 
Description The present investigation has been undertaken to: (i) carry out field evaluation of various Basmati
(Pusa1121, HBC19 and Pusa Basmati 1460), indica (PAU201, HKR47) and aerobic (MAS-ARB25, MASARB868,
MAS25, MAS26 and MAS109,) rice varieties for various agronomic traits under submerged field
conditions, (ii) develop F1 and/or backcross plants from crosses between aerobic and high yielding rice
cultivars, and (ii) assess the molecular status of F1 and parental rice genotypes for some markers linked
with the root traits, abiotic stress tolerance and BAD2 aroma gene. Aerobic rice varieties had small panicle
length and low yield per plant compared to Basmati and high yielding indica rice varieties when grown
under submerged cultivations in the field. A DNA fingerprinting database of ten parental genotypes has
been prepared using 29 SSR markers and a BAD2 aroma gene specific marker. High level of polymorphism
was observed among the aerobic, Basmati and indica rice varieties. A total of 117 alleles were detected at
30 loci. Number of alleles per locus ranged from 2 to 6 with an average of 3.9 alleles per locus. The overall
size of PCR products amplified range from 70-585 bp. The molecular size difference between the smallest
and largest allele at a locus varied from 2 to 328. The polymorphism information content (PIC) values,
which are indicative of genetic diversity, ranged from 0.180 to 0.820 with an overall average of 0.616 per
locus. The NTSYS-PC UPGMA tree cluster as well as PCA analysis clearly grouped the 10 rice genotypes
into two major groups one with three Basmati rice cultivars and other with aerobic as well as indica rice
genotypes. Polymorphism for seven microsatellite markers (RM205, RM206, RM242, RM440, RM447,
RM562 and RM7075) and BAD2 aroma gene specific (Bradbury et al. 2005) markers with a base
difference of >30 bp in the amplified products, could be clearly visualized on 2.5% w/v agarose gels. The
two F1 plants HBC19 x MAS26 and Pusa1121 x MAS25 showed lower levels of seed setting (8.0 and
20.6%, respectively) that too only in end of October and starting of November. No backcrossed seed was
obtained. A total of 15 crosses were made between aerobic and high yielding indica /Basmati rice varieties;
crossed seed were recovered from 11 crosses. Seven plants obtained from the HKR47 x MAS26 and MASARB25
x Pusa Basmati crosses were confirmed as F1 hybrids with the help of SSR RM242 and BAD2
aroma gene specific markers.
 
Date 2016-11-11T12:11:35Z
2016-11-11T12:11:35Z
2010
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/85076
 
Language en
 
Format application/pdf
 
Publisher CCSHAU