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Studies on molecular variations in Phaeoisoropsis personata (Berk and M.A. Curtis) van Arx. Causing late leaf spot of groundnut (Arachis hypogea L.)

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Title Studies on molecular variations in Phaeoisoropsis personata (Berk and M.A. Curtis) van Arx. Causing late leaf spot of groundnut (Arachis hypogea L.)
 
Creator Kumari
 
Contributor S.S.Adiver
 
Subject Plant pathology
 
Description The present investigation on molecular variability in Phaseoisariopsis personata was
carried out during the period 2006-08 at the Department of Plant Pathology, College of
Agriculture, University of Agricultural Sciences, Dharwad. The study includes molecular
variability in P. personata through isozyme and RAPD technique. Pathogenic isolates of P.
personata were obtained from 15 commonly grown groundnut cultivars (resistant and
susceptible genotypes) in MARS, UAS Dharwad and also collected in nine locations of
Karnataka (Arabhavi, Annigere, Bijapur, Indalgi, Hammanamatti, Shirahatti, Nippani,
Raichur and Dharwad) for investigation on molecular variability. Based on isozyme analysis
of four enzymes (peroxidase, Polyphenol oxidase, catalase and super-oxide-dismutase) two
major clusters were obtained which revealed little variation in their protein makeup. Based on
isozyme bands it was possible to categorize the isolates into different groups. From the
investigation it is clear that the isolates V14 (LSVT-1-2006-2) and HAN (Hanumanamatti)
exhibited more peroxidase activity followed by isolates V15 (TAG-24) and IND (Indalgi)
from Dharwad and other locations respectively by expressing more number of bands. Based
on RAPD data distinguished the fifteen isolates from Dharwad and eight isolates from other
locations into two major clusters, A and B each. Among the isolates from Dharwad location,
isolates V12 (ICGV-86950) and V13 (LSVT-I-2005-7) had maximum similarity, whereas
isolates V8 (JL-24) and V1 (DH-212) showed least similarity. Among the isolates obtained
from different locations; isolates from Annigere and Bijapur had maximum similarity
whereas isolates from Raichur and Arabhavi showed least similarity. So the results obtained
from the cluster analysis revealed that sub-cluster groups composed of isolates belonging to
same geographical locations with certain variability. The present investigation revealed a
great molecular variation existing among the isolates of P. personata which could be used to
distinguish variation among the isolates of P. personata. The study also brought out that, the
pathogen showed molecular variation over locations and also in a location depending on the
genotypes grown there.
 
Date 2016-10-27T18:40:42Z
2016-10-27T18:40:42Z
2008
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/82619
 
Format application/pdf