PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF GUAR (Cyamopsis tetragonoloba L.) GENOTYPES FOR DROUGHT TOLERANCE
KrishiKosh
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Title |
PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF GUAR (Cyamopsis tetragonoloba L.) GENOTYPES FOR DROUGHT TOLERANCE
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Creator |
ANUPAM CHAKRABORTY
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Contributor |
SUDHAKAR, P
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Subject |
PHYSIOLOGICAL, MOLECULAR, CHARACTERIZATION, GUAR, GENOTYPES, DROUGHT, TOLERANCE
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Description |
Field experiments were conducted during late Rabi 2006-07 on “Physiological and Molecular characterisation of guar (Cyamopsis tetragonoloba L.) genotypes for drought tolerance” at college farm of College of Agriculture, Rajendranagar, Hyderabad. There were two main treatments i.e. irrigated and simulated mid season moisture stress (35 DAS to 70 DAS)]. The sub treatments consisted of 10 guar genotypes. The treatments were arranged in a split plot design and replicated thrice. The results revealed that significant differences between irrigation treatments and genotypes were observed for dry matter accumulation and leaf area, only after imposition of moisture stress at 35 DAS. Similar results were also observed with physiological traits viz., leaf area index, crop growth rate and net assimilation rate. Among the ten genotypes tested, WSP-50, GG-1 and RGM-111 maintained higher values for these traits especially under moisture stress imposed treatment throughout the crop growth. Among the drought tolerance traits used for characterisation, SPAD chlorophyll meter readings (SCMR) and leaf peroxidase activity established a positive correlation and specific leaf area showed a negative correlation with seed yield. A negative correlation was also recorded between specific leaf area and SCMR under moisture stress condition. Leaf relative water contents were significantly reduced by 35% in moisture stress treatment compared to irrigated control. The genotypes WSP-50, GG-1 and RGM-111 recorded lower SLA, higher SCMR, RWC values with higher peroxidase activity under moisture stress condition, which establish the drought tolerance nature of these genotypes compared to other entries. Further, these genotypes matured early possessing drought avoidance mechanism. Earliness also showed a positive correlation with seed yield in limited moisture conditions. Yield components i.e. number of clusters/plant, number of seed/pod, seed yield varied significantly between irrigation treatments, such effect was not observed among the genotypes. 100 seed weight, and harvest index did not differ significantly between irrigation treatments and genotypes. The genotypes WSP-50, GG-1 and RGM-111 maintained higher seed yields under moisture stress condition. Vikas-35, RGC-1038, HGS884, GUAG-OO3, RGC-9708 recorded moderate yields and GUAG- 9808, GUAG-9708 recoded lower yields. Protein profiling was done with SDS-PAGE. Under moisture stress condition an accumulated protein of molecular weight 55 kd was observed to be down regulated for osmoregulation to acquire drought tolerance in all the genotypes, however band intensity varied between genotypes. High amount of DNA polymorphism (73.5%) was also observed between the ten guar genotypes, which is a prime thing to develop DNA marker, to use in Marker Assisted Selection breeding programmes. Genetic similarities were also established between these genotypes. Using cluster analysis and dendrogram results, the following clusters are formed. a) GAUG 9708, Vikas-35, GG-1 – Distant and Diverse b) RGC-1078, RGM-111- 90.9% similarity c) GUAG-9808, GUAG-003- 78.6% similarity d) RGC-1038, HGS-884, WSP-50.- 71.9%-75.8% similarity |
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Date |
2016-08-05T14:29:25Z
2016-08-05T14:29:25Z 2007 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/71077
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Language |
en
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Relation |
D8111;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD
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