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PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF GUAR (Cyamopsis tetragonoloba L.) GENOTYPES FOR DROUGHT TOLERANCE

KrishiKosh

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Title PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION OF GUAR (Cyamopsis tetragonoloba L.) GENOTYPES FOR DROUGHT TOLERANCE
 
Creator ANUPAM CHAKRABORTY
 
Contributor SUDHAKAR, P
 
Subject PHYSIOLOGICAL, MOLECULAR, CHARACTERIZATION, GUAR, GENOTYPES, DROUGHT, TOLERANCE
 
Description Field experiments were conducted during late Rabi 2006-07 on “Physiological
and Molecular characterisation of guar (Cyamopsis tetragonoloba L.) genotypes for
drought tolerance” at college farm of College of Agriculture, Rajendranagar,
Hyderabad. There were two main treatments i.e. irrigated and simulated mid season
moisture stress (35 DAS to 70 DAS)]. The sub treatments consisted of 10 guar
genotypes. The treatments were arranged in a split plot design and replicated thrice.
The results revealed that significant differences between irrigation treatments
and genotypes were observed for dry matter accumulation and leaf area, only after
imposition of moisture stress at 35 DAS. Similar results were also observed with
physiological traits viz., leaf area index, crop growth rate and net assimilation rate.
Among the ten genotypes tested, WSP-50, GG-1 and RGM-111 maintained higher
values for these traits especially under moisture stress imposed treatment throughout
the crop growth.
Among the drought tolerance traits used for characterisation, SPAD
chlorophyll meter readings (SCMR) and leaf peroxidase activity established a positive
correlation and specific leaf area showed a negative correlation with seed yield. A
negative correlation was also recorded between specific leaf area and SCMR under
moisture stress condition. Leaf relative water contents were significantly reduced by
35% in moisture stress treatment compared to irrigated control.
The genotypes WSP-50, GG-1 and RGM-111 recorded lower SLA, higher
SCMR, RWC values with higher peroxidase activity under moisture stress condition,
which establish the drought tolerance nature of these genotypes compared to other
entries. Further, these genotypes matured early possessing drought avoidance
mechanism. Earliness also showed a positive correlation with seed yield in limited
moisture conditions.
Yield components i.e. number of clusters/plant, number of seed/pod, seed
yield varied significantly between irrigation treatments, such effect was not observed
among the genotypes. 100 seed weight, and harvest index did not differ significantly
between irrigation treatments and genotypes. The genotypes WSP-50, GG-1 and
RGM-111 maintained higher seed yields under moisture stress condition. Vikas-35,
RGC-1038, HGS884, GUAG-OO3, RGC-9708 recorded moderate yields and GUAG-
9808, GUAG-9708 recoded lower yields.
Protein profiling was done with SDS-PAGE. Under moisture stress condition
an accumulated protein of molecular weight 55 kd was observed to be down regulated
for osmoregulation to acquire drought tolerance in all the genotypes, however band
intensity varied between genotypes.
High amount of DNA polymorphism (73.5%) was also observed between the
ten guar genotypes, which is a prime thing to develop DNA marker, to use in Marker
Assisted Selection breeding programmes. Genetic similarities were also established
between these genotypes. Using cluster analysis and dendrogram results, the
following clusters are formed.
a) GAUG 9708, Vikas-35, GG-1 – Distant and Diverse
b) RGC-1078, RGM-111- 90.9% similarity
c) GUAG-9808, GUAG-003- 78.6% similarity
d) RGC-1038, HGS-884, WSP-50.- 71.9%-75.8% similarity
 
Date 2016-08-05T14:29:25Z
2016-08-05T14:29:25Z
2007
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/71077
 
Language en
 
Relation D8111;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD