DEVELOPMENT OF MOLECULAR IDs FOR ELITE INDIAN RICE VARIETIES AND MARKER-BASED ASSESSMENT OF SEED PURITY
KrishiKosh
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Title |
DEVELOPMENT OF MOLECULAR IDs FOR ELITE INDIAN RICE VARIETIES AND MARKER-BASED ASSESSMENT OF SEED PURITY
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Creator |
MRINALI M. MANDAPE
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Contributor |
KESHAVULU, K
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Subject |
rice, genetics, sowing, purity, planting, dna, alleles, genomes, polymorphism, genetic markers
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Description |
The present investigation was undertaken with an objective to characterize elite Indian rice varieties with the help of molecular markers and develop their molecular ID andalso to analyze the genetic purity of seeds of the selected elite rice varieties in different seed lots (seeds collected at various stages of seed multiplication) through Grow-out Test (GOT) and molecular marker-based assays. The study also intended to compare the results of GOT and molecular marker-based assay and demonstrate the utility of molecular markers in the assessment of purity in seed-lots.The material for investigation comprised offifteen elite rice varieties for the development of molecular IDs and four varieties viz., MTU 1010, MTU 7029, BPT 5204 and Improved Samba Mashuri for genetic purity analysis and validation of genotype specific markers. The study was conducted at Directorate of Rice Research, Rajendranagar, Hyderabad during 2013-14. A total of 48 SSR markers distributed across the 12 rice chromosomes were employed for the study. Out of them 30 markers were observed to be polymorphic producing 69 alleles which amplified among the fifteen rice genotypes analysed. The Polymorphic Information Content (PIC) value ranged from 0.03 to 0.64. The primers RM24888 with an allele size of 200 bp uniquely identified the variety MTU 1010 from others. The marker combination of RM206 and ESSR12- 20.2 with an allele size of 450 bp and 170 bp respectively, identified the variety MTU 7029 uniquely. The marker combination of RM10001 and RM206 with allele size of 480 bp and 450 bp respectively, identified the variety BPT 5204 uniquely. The marker combination of JGT06-6.81 and RM22266 with allele size of 450 bp and 200 bp respectively, identified the variety Improved Samba Mashuri uniquely. Thus, these marker or marker combination may serve as molecular IDs. When the foundation and certified seed lots (truthfully labeled in case of Improved Samba Mashuri) were assessed for genetic purity through GOT and molecular markers, impurity percentage detected through molecular markers was 0.75 to 2.75% indicating the superiority of assays based on molecular markers especially EST-SSRs over GOT. Also the results demonstrated a marked decrease in the genetic purity in subsequent classes of seedviz., foundation, certified and truthfully labeled seeds which can be assessed rapidly and efficiently by molecular marker-based assays. |
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Date |
2016-09-14T13:34:22Z
2016-09-14T13:34:22Z 2014 |
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Type |
Thesis
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Identifier |
http://krishikosh.egranth.ac.in/handle/1/76681
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Language |
en
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Relation |
D9727;
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Format |
application/pdf
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Publisher |
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD
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