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DEVELOPMENT OF MOLECULAR IDs FOR ELITE INDIAN RICE VARIETIES AND MARKER-BASED ASSESSMENT OF SEED PURITY

KrishiKosh

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Title DEVELOPMENT OF MOLECULAR IDs FOR ELITE INDIAN RICE VARIETIES AND MARKER-BASED ASSESSMENT OF SEED PURITY
 
Creator MRINALI M. MANDAPE
 
Contributor KESHAVULU, K
 
Subject rice, genetics, sowing, purity, planting, dna, alleles, genomes, polymorphism, genetic markers
 
Description The present investigation was undertaken with an objective to characterize
elite Indian rice varieties with the help of molecular markers and develop their
molecular ID andalso to analyze the genetic purity of seeds of the selected elite rice
varieties in different seed lots (seeds collected at various stages of seed
multiplication) through Grow-out Test (GOT) and molecular marker-based assays.
The study also intended to compare the results of GOT and molecular marker-based
assay and demonstrate the utility of molecular markers in the assessment of purity in
seed-lots.The material for investigation comprised offifteen elite rice varieties for the
development of molecular IDs and four varieties viz., MTU 1010, MTU 7029, BPT
5204 and Improved Samba Mashuri for genetic purity analysis and validation of
genotype specific markers. The study was conducted at Directorate of Rice Research,
Rajendranagar, Hyderabad during 2013-14.
A total of 48 SSR markers distributed across the 12 rice chromosomes were
employed for the study. Out of them 30 markers were observed to be polymorphic
producing 69 alleles which amplified among the fifteen rice genotypes analysed. The
Polymorphic Information Content (PIC) value ranged from 0.03 to 0.64.
The primers RM24888 with an allele size of 200 bp uniquely identified the
variety MTU 1010 from others. The marker combination of RM206 and ESSR12-
20.2 with an allele size of 450 bp and 170 bp respectively, identified the variety
MTU 7029 uniquely. The marker combination of RM10001 and RM206 with allele
size of 480 bp and 450 bp respectively, identified the variety BPT 5204 uniquely.
The marker combination of JGT06-6.81 and RM22266 with allele size of 450 bp and
200 bp respectively, identified the variety Improved Samba Mashuri uniquely. Thus,
these marker or marker combination may serve as molecular IDs.
When the foundation and certified seed lots (truthfully labeled in case of
Improved Samba Mashuri) were assessed for genetic purity through GOT and
molecular markers, impurity percentage detected through molecular markers was
0.75 to 2.75% indicating the superiority of assays based on molecular markers
especially EST-SSRs over GOT. Also the results demonstrated a marked decrease in
the genetic purity in subsequent classes of seedviz., foundation, certified and
truthfully labeled seeds which can be assessed rapidly and efficiently by molecular
marker-based assays.
 
Date 2016-09-14T13:34:22Z
2016-09-14T13:34:22Z
2014
 
Type Thesis
 
Identifier http://krishikosh.egranth.ac.in/handle/1/76681
 
Language en
 
Relation D9727;
 
Format application/pdf
 
Publisher ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABAD